Share This Article:

Technique to Obtain Mitotic Chromosomes of Conyza bonariensis L. Cronquist (Asteraceae)

Abstract Full-Text HTML XML Download Download as PDF (Size:453KB) PP. 1466-1474
DOI: 10.4236/ajps.2015.69145    3,670 Downloads   4,254 Views   Citations

ABSTRACT

Cytogenetic analysis requires cytological preparations that unequivocally reveal the chromosome number and permit optimal visualizations of chromosome morphology for the construction of karyotypes and ideograms. Chromosomal characterization is possible only by establishing these two parameters. To cytogenetically characterize the weed Conyza bonariensis (L.) Cronquist (Asteraceae), it was necessary to improve cytological analysis techniques to obtain optimal results. This species belongs to a genus whose plants have wide phenotypic plasticity. These plants can be morphologically differentiated by other types of analysis, and thus the application of this technique will serve as a reference for cytogenetical analysis of other groups of plants that have cytogenetic characteristics similar to those of C. bonariensis. The methodology described here highlights three main protocol steps: 1) root tip collection from newly germinated seed radicles and from young root tips of mature plants, 2) pretreatment of meristems with antimitotic agents, both isolated and combined, and 3) acid and enzymatic hydrolytic processes.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

de Paula, J. and Ferreira Pinto-Maglio, C. (2015) Technique to Obtain Mitotic Chromosomes of Conyza bonariensis L. Cronquist (Asteraceae). American Journal of Plant Sciences, 6, 1466-1474. doi: 10.4236/ajps.2015.69145.

References

[1] Sharma, A.K. and Sharma, A. (2014) Chromosome Techniques: Theory and Practice. Butterworth-Heinemann, Oxford, 724 p.
[2] Nascimento, H.R.do, Gallo, R., Karsburg, I.V. and Oliveira, A.S. (2014) Cytogenetic and Identification of the Nucleolus Organizer Region in Heliconia bihai (L.). Revista Ceres, 61, 451-457.
http://dx.doi.org/10.1590/0034-737X201461040002
[3] Singha, H.R., Debbarma, S., Sinha, J., Sinha, S. and Sinha, R.K. (2015) Karyomorphological Study of Blumea aromatica DC: An Ethnobotanical Species of Manipuri Community of Tripura, India. Asian Journal of Plant Science and Research, 5, 18-21.
[4] Mohajer, S. and Taha, R.M. (2014) Observations on the Cytology and Karyogram of an Onobrychis viciifolia Scop. New Variety in Callus, in Vivo and in Vitro Cultures. Journal of Agricultural Science and Technology, 16, 1683-1698.
[5] Razaq, Z.A., Vahidy, A.A. and Ali, S.I. (1994) Chromosome Numbers in Compositae from Pakistan. Annals of the Missouri Botanical Garden, 81, 800-808.
http://dx.doi.org/10.2307/2399925
[6] Ostergren, G. and Heneen, W.K. (1962) A Squash Technique for Chromosome Morphological Studies. Hereditas, 48, 332-341.
http://dx.doi.org/10.1111/j.1601-5223.1962.tb01817.x
[7] Vargas, L., Bianchi, M.A., Rizzardi, M.A., Agostinetto, D. and Dal Magro, T. (2007) Buva (Conyza bonariensis) resistente ao glyphosate na Região Sul do Brasil. Planta Daninha, 25, 573-578.
http://dx.doi.org/10.1590/S0100-83582007000300017
[8] Lamego, F.P. and Vidal, R.A. (2008) Resistência ao glyphosate em biótipos de Conyza bonariensis e Conyza canadensis no Estado do Rio Grande do Sul, Brasil. Planta Daninha, 26, 467-471.
http://dx.doi.org/10.1590/S0100-83582008000200024
[9] Moreira, M.S., Nicolai, M., Carvalho, S.J.P. and Christoffoleti, P.J. (2007) Resistência de Conyza canadensis e C. bonariensis ao herbicidaglyphosate. Planta Daninha, 25, 157-164.
http://dx.doi.org/10.1590/S0100-83582007000100017
[10] Santos, G., Oliveira Jr., R.S., Constantin, J., Francischini, A.C. and Osipe, J.B. (2014) Multiple Resistance of Conyza sumatrensis to Chlorimuron-Ethyl and to Glyphosate. Planta Daninha, 32, 409-416.
http://dx.doi.org/10.1590/S0100-83582014000200019
[11] Powell, A.M. and Turner, B.L. (1963) Chromosome Numbers in the Compositae. VII. Additional Species from the Southwestern United States and Mexico. Madroño, 17, 128-140.
[12] Solbrig, O.T. (1964) Chromosome Number in Compositae V. Astereae II. American Journal of Botany, 51, 513-519.
http://dx.doi.org/10.2307/2440279
[13] Solbrig, O.T., Anderson, L.C., Kyhos, D.W. and Raven, P.H. (1969) Chromosome Numbers in Compositae VII: Astereae III. American Journal of Botany, 56, 348-353.
http://dx.doi.org/10.2307/2440859
[14] Powell, A.M. and King, R.M. (1969) Chromosome Numbers in the Compositae: Colombian Species. American Journal of Botany, 56, 116-121.
http://dx.doi.org/10.2307/2440402
[15] Powell, A.M. and King, R.M. (1969) Chromosome Numbers in the Compositae: West Indian Species. Contributions to Botany, 3, 319-320.
[16] Urdampilleta, J.D., Amat, A.G. and Bidau, C.J. (2005) Karyotypic Studies and Morphological Analysis of Reproductive Features in Five Species of Conyza (Astereae: Asteraceae) from Northeastern Argentina. Boletín de la Sociedad Argentina de Botanica, 40, 91-99.
[17] Turner, B.L., Bacon, J., Urbatsch, L. and Simpson, B. (1979) Chromosome Numbers in South American Compositae. American Journal of Botany, 66, 173-178.
http://dx.doi.org/10.2307/2442521
[18] Wulff, A.F. (1998) Estudios cariológicos en Asteraceae VIII. Darwiniana, 35, 37-43.
[19] Carr, G.D., King, R.M., Powell, A.M. and Harold, R. (1999) Chromosome Numbers in Compositae. XVIII. American Journal of Botany, 86, 1003-1013.
http://dx.doi.org/10.2307/2656618
[20] Guareschi, A., Pastori, T., Kruse, N.D., Machado, S.L.O., do Dorow, T.S. and Tedesco, S.B. (2012) Cytogenetics Characterization of Conyza bonariensis (L.) Cronquist Populations from Brazil. Ciência e Natura, 34, 39-48.
http://dx.doi.org/10.5902/2179460X9353
[21] Jansen, R.K. and Stuessy, T.F. (1980) Chromosome Counts of Compositae from Latin America. American Journal of Botany, 67, 585-594.
http://dx.doi.org/10.2307/2442300
[22] Jansen, R.K., Stuessy, T.F., Dias-Piedrahit, S. and Funk, V.A. (1984) Recuentos cromosómicos en Compositae de Colombia. Caldasia, 14, 7-20.
[23] Bernardello, L.M. (1986) Números cromosómicos en Asteraceae de Córdoba. Darwiniana, 27, 169-178.
[24] Guerra, M. and Souza, M.J. (2002) Como observar cromossomos: Um guia de técnica em citogenética vegetal, animal e humana. Funpec, São Paulo, 131 p.
[25] Singh, R.J. (2003) Plant Cytogenetics. CRC Press, Boca Raton, 488 p.
[26] Conagin, C.H.T.M. (1972) Colchicine Effects on Arachis hypogaea L. Bragantia, 31, 187-198.
http://dx.doi.org/10.1590/S0006-87051972000100015
[27] Tognon, G.B., Pimenta, A.C., Panobianco, M., Cuquel, F.L. and Zuffellato-Ribas, K.C. (2014) Germinação e morfologia de diásporos e plantulas de Bidens segetum Mart. ex Colla. Revista Agro@mbiente on line, 8, 238-243.
[28] Tlaskal, J. (1980) Combined Cycloheximide and 8-Hydroxyquinoline Pretreatment for Study of Plant Chromosomes. Stain Technology, 54, 313-319.
http://dx.doi.org/10.3109/10520297909110690
[29] El-esawi, A. and Sammour, R. (2014) Karyological and Phylogenetic Studies in the Genus Lactuca L. (Asteraceae). Cytologia, 79, 269-275.
http://dx.doi.org/10.1508/cytologia.79.269
[30] Via do Pico, G.M. and Dematteis, M. (2014) Cytotaxonomy of Two Species of Genus Chrysolaena H. Robinson, (Vernonieae, Asteraceae) from Northeast Paraguay/Comparative. Cytogenetics, 8, 125-137.
[31] Tatarenko, E., Smirnov, S.V. and Kondo, K. (2013) Chromosome Relations Hips among the Members of Chrysanthemum fruticulosum Complex II. Hexaploid Strain of Artemisia obtusiloba in Altai Mts., Russia. Chromosome Botany, 8, 45-46.
http://dx.doi.org/10.3199/iscb.8.45
[32] Sato, K., Yamazaki, T. and Iwatsubo, Y. (2012) Karyotypes of Taraxacum laevigatum (Asteraceae) in Japan. Cytologia, 77, 211-214.
http://dx.doi.org/10.1508/cytologia.77.211
[33] Torres, A.M. and Liogier, A.H. (1970) Chromosome Numbers of Dominican Compositae. Brittonia, 22, 240-245.
http://dx.doi.org/10.2307/2805906
[34] Baltisberger, M. and Widmer, A. (2006) Chromosome Numbers of Plant Species from the Canary Islands. Botanica Helvética, 116, 9-30.
http://dx.doi.org/10.1007/s00035-006-0739-x

  
comments powered by Disqus

Copyright © 2019 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.