Determination of Salmonella pullorum with Nanoparticles Immune Based Lateral Flow Strip Assay

Bin Wu^1*, Xiuyun Zhang¹, Wei Pan², Lin Zhang², Fan Zhang²

¹Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian, China.
²Chinese Academy of Inspection and Quarantine, Beijing, China.

DOI: 10.4236/aim.2015.55038                

Abstract

The isolation and culture of conventional detection method of salmonella can not meet the testing requirements of quick and easy detection at the grassroots level. In this study, we prepare the fluorescent nanoparticles as a marker, covalently conjugate with monoclonal antibodies of Salmonella pullorum. The whole Salmonella pullorum antigen and goat anti-mouse antibody sprayed on the nitrocellulose membranes are used as test line and control line. The fluorescence nanoparticles immune based lateral flow strips are made according to the principle of antigen-antibody immune response. The test strips may interpret results within 30 min. The results of the salmonella A, S. agona, S. chester and S. arechavaleta are positive, including, S. agona for weakly positive. After analysis, it is found that in addition to the salmonella of group A, the other positive salmonella are in group B. But it is negative of S. derby, S. rissen, and other 6 kinds of salmonella, with good specificity. The fluorescence nanoparticles immune based lateral flow strips are a little of sample can be detected fast, easily, inexpensive, easy to universal without professional technical personnel detection method. It provides a new detection method for the detections of Salmonella pullorum.

Keywords

Monoclonal Antibody, Nanoparticles, Fluorescence Nanoparticles Immune Based Lateral Flow Strips, Salmonella pullorum

Conflicts of Interest

The authors declare no conflicts of interest.

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