Phenotypic Characterization Murine Sarcoma TG-180 Immunophenotypical Characterization Murine Sarcoma TG-180

Thais M. T. Bernardes¹, Hugo E. O. Beserra¹, Stephane C. O. R. Vexenat¹, Helio Langoni², Noeme S. Rocha^1*

¹Department of Clinical Veterinary Medicine, Laboratory of Investigative and Comparative Pathology, School of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, Brazil.
²Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, S?o Paulo State University, Botucatu, Brazil.

DOI: 10.4236/ojpathology.2015.52009                

Abstract

The sarcoma is the generic nomenclature for neoplasm of mesodermal cells, which express in man and animals. Silent growth requires early diagnosis technique for identifying their proteins. The experimental model in vivo murine sarcoma 180-TG (TG-180), is widely used in research to provide the stimuli of infectious and neoplastic antigens. In this case, the technique of immunohisto-chemistry helps identify the expressions of tumor cell variants. The objective of the research was to characterize immunoexpression murine sarcoma TG 180, by immunohistochemistry, antibodies AE1/AE3, vimentin, CD3, CD 45, CD79α and S100A4. For this, murine sarcoma TG-180, was implanted subcutaneously in 20 mice “Swiss”, male, 30 days old, 28 g for 10 days. Samples were taken and subjected to immunohistochemistry, via use of HistoMouse-MA™? kit. There was specifically labeled S100A4 and vimentin antibodies, indicative of poorly differentiated neoplasms fibroblasts. In fact, the model is established by identifying the origin of the cell, once identified, chemotherapeutic tests can also be performed. Neoplasia like these, when installed in man and animals, depending on the degree of aggressiveness requires treatment protocol varied between surgery and chemotherapy or combination of treatments.

Keywords

Immunohistochemistry, Neoplasia, Mice, Tumor Origin

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Schor, M. and Boim, A.M. (2011) Importancia do uso de animais experimentais para a medicina humana. Arquivo brasileiro de ciência da saúde, 33, 7.
[2]	Assef, M.L.M., Carneiro-Leão, A.M., Moretão, M.P., Azambuja, A.P., Iacomini, M. and Buchi, D.F. (2002) Histological and Immunohistochemical Evaluation of Sarcoma 180 in Mice after Treatment with an α-d-Glucan from the Lichen Ramalina Celastri. Brazilian Journal. Morphology Science, 19, 49-54.
[3]	Florêncio, A.P.S., Melo, J.H.L., Mota, C.R.F.C., Melo Jr., M.R. and Araújo, R.V.S. (2007) Estudo da atividade anti- tumoral do polissacarídeo (PJU) extraído de Anacardium occidentale frente a um modelo experimental do sarcoma 180. Revista Eletronica de Farmácia, 4, 61-65.
[4]	Rehg, J.E. and Ward, J.M. (2012) Morphological and Immunohistochemical Characterization of Sarcomatous Tumors in Wild-Type and Genetically Engineered Mice Veterinary. Pathology, 49, 206-207.
[5]	Machado, M.C.F.P. and Melo-júnior, M.R. (2009) Avaliação do efeito antitumoral da kalanchoe brasiliensis sobre o sarcoma 180 em camundongos. Revista Eletrônica de Farmácia, 6, 1-6.
[6]	Qi, L. and Xu, Z. (2006) In Vivo Antitumor Activity of Chitosan Nanoparticles. Bioorganic Medicinal Chemistry Letters, 16, 4243-4245. http://dx.doi.org/10.1016/j.bmcl.2006.05.078
[7]	Zuckerberg, C. (1973) Ultrastructure of Sarcoma 180. Cancer Research, 33, 2278-2282.
[8]	Riggi, N., Cironi, L., Suvá, M.L. and Stamenkovic, I. (2007) Sarcomas: Genetics, Signalling, and Cellular Origins. Part 1: The Fellowship of TET. Journal Pathology, 9, 4-20. http://dx.doi.org/10.1002/path.2209
[9]	Ettinger, S.N. (2003) Principles of Treatment for Soft-Tissue Sarcomas in the Dog. Clinical Technology Small Animal Practice, 18, 118-122. http://dx.doi.org/10.1053/svms.2003.36628
[10]	Bezerra, D.P., Castro, F.O., Alves, A.P.N.N., Pessoa, C., Moraes, M.O. and Silveira, E.R. (2006) In Vivo Growth-Inhibition of Sarcoma 180 by Piplartine and Piperine, Two Alkaloid Amides from Piper. Brazilian Journal Medical Biology Research, 39, 801-807. http://dx.doi.org/10.1590/S0100-879X2006000600014
[11]	Castro, A.P.B., Silva, R.C. and Langoni, H. (2008) Produção de antígenos de Toxoplasma gondii em células de sarcoma murino TG180. Veterinaria e Zootecnia, 15, 288-298.
[12]	Wick, M.R. (2008) Immunohistochemical Approaches to the Diagnosis of Undifferentiated Malignant Tumors. Annals of Diagnostic Pathology, 12, 72-84. http://dx.doi.org/10.1016/j.anndiagpath.2007.10.003
[13]	Pires, W.C., Mello, F.M.S., Batista, M.P., Pereira, F.C., Lima, A.P. and Vilanova-Costa, C.A.S.T. (2011) Estudo da atividade citotóxica do extrato bruto etanólico de Psychotria prunifolia (Rubiaceae) em células tumorais e normais in vitro. Revista de Biologia Neotropical, 8, 15-23.
[14]	Zhang, J., Chen, L., Liu, X., Kammertoens, T., Blankenstein, T. and Gin, Z. (2013) Fibroblast-Specific Protein 1/S100A4-Positive Cells Prevent Carcinoma through Collagen Production and Encapsulation of Carcinogens. Cancer Research, 1, 2770-2781. http://dx.doi.org/10.1158/0008-5472.CAN-12-3022