Physiological Response of Mung Bean to Polyethylene Glycol Drought Stress at Flowering Period

Zhichao Yin¹, Jie Liang², Xiyu Hao², Huan Lu¹, Jianjun Hao^1*, Fengxiang Yin^2*

¹Biotechnology College, Shenyang Agricultural University, Shenyang, China.
²Baicheng Academy of Agricultural Sciences, Baicheng, China.

DOI: 10.4236/ajps.2015.65084                

Abstract

The study was carried out on the basis of the flowering period of mung bean (Phaseolus radiatus). The polyethylene glycol 6000 (PEG6000) was used to stimulate water stress. Four mung bean varieties with different drought resistances were used as materials in this experiment, in which the physiological indexes of mung bean for the identification of drought resistance were selected, and the response analysis of seven physiological indexes to different degrees of drought stress was measured by using polyethylene glycol 6000 (PEG6000) on different concentrations of 5%, 10% and 20%. The physiological mechanism was analysed on drought tolerance of mung beans. This test was to set up identification system for drought tolerance of flowering mung bean. The results showed that the relative conductivity after 6 days of 5% PEG treatment, the SOD activity after 6 days of 5% PEG treatment, the POD activity after 6 days of 10% PEG treatment and the ABA content after 6 days of 10% PEG treatment could be used as drought-tolerant identification for mung beans at flowering period. These four physiological indexes and drought-resistant index weighted grey correlation analysis results showed that the correlation order was successively SOD activity (0.8589) > ABA content (0.8290) > conductivity (0.7160) > POD activity (0.6637); and the grey correlation degree was greater than 0.6500 among the four physiological indexes.

Keywords

Mung Bean, Flowering, Drought, PEG, Physiological Index

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Fu, G.-F., Song, J., Xiong, J., Li, Y.-R., Chen, H.-Z., Le, M.-K. and Tao, L.-X. (2011) Changes of Oxidative Stress and Soluble Sugar in Anthers Involve in Rice Pollen Abortion under Drought Stress. Agricultural Sciences in China, 10, 1016-1025. http://dx.doi.org/10.1016/S1671-2927(11)60089-8
[2]	Wan, F. (2006) World Overwintering Winter Wheat Improvement Theory and Practice. Agricultural Science and Technology Press, Beijing.
[3]	Nielsen, D.C., Vigil, M.F. and Benjamin, J.G. (2009) The Variable Response of Dry Land Corn Yield to Soil Water Content at Planting. Agricultural Water Management, 96, 330-336. http://dx.doi.org/10.1016/j.agwat.2008.08.011
[4]	Bouchenak, M. and Lamri-Senhadji, M. (2013) Nutritional Quality of Legumes, Their Role in Cardiometabolic Risk Prevention. Journal of Medicinal Food, 16, 185-198. http://dx.doi.org/10.1089/jmf.2011.0238
[5]	Anjum, S.A., Farooq, M., Xie, X.-Y., Liu, X.-J. and Ijaz, M.F. (2012) Antioxidant Defense System and Proline Accumulation Enables Hot Pepper to Perform Better under Drought. Scientia Horticulturae, 140, 66-73. http://dx.doi.org/10.1016/j.scienta.2012.03.028
[6]	Hao, J.J., Lu, W., Huang, C.H., Yu, Y. and Yin, F.X. (2013) Different Physicochemical Properties of Mung Bean Varieties Comparison. Agricultural Sciences—Jilin, China, 38, 19-21, 42.
[7]	Hao, J.J. and Li, K.Z. (2005) Plant: Physiology Experimental Guide. Chemical Industry Press, China.
[8]	Li, C.-N., Srivastava, M.-K., Nong, Q., Yang, L.-T. and Li, Y.-R. (2013) Molecular Cloning and Characterization of SoNCED, A Novel Gene Encoding 9-Cis-Epoxycarotenoid Dioxygenase from Sugarcane (Saccharum officinarum L.). Genes Genomics, 35, 101-109. http://dx.doi.org/10.1007/s13258-013-0065-9
[9]	Ding, Z.-S., Zhou, B.-Y., Sun, X.-F. and Zhao, M. (2012) High Light Tolerance Is Enhanced by Overexpressed PEPC in Rice under Drought Stress. Acta Agronomica Sinica, 38, 285-292. http://dx.doi.org/10.1016/S1875-2780(11)60106-5
[10]	Silva, E.N., Ribeiro, R.V., Ferreira-Silva, S.L., Vieira, S.A., Ponte, L.F.A. and Silveira, J.A.G. (2012) Coordinate Changes in Photosynthesis, Sugar Accumulation and Antioxidative Enzymes Improve the Performance of Jatropha curcas Plants under Drought Stress. Biomass and Bioenergy, 45, 270-279. http://dx.doi.org/10.1016/j.biombioe.2012.06.009
[11]	Borde, M., Dudhane, M. and Jite, P. (2012) Growth, Water Use Efficiency and Antioxidant Defense Responses of Mycorrhizal and Non Mycorrhizal Allium sativum L. under Drought Stress Condition. Annals of Plant Sciences, 1, 6-11.
[12]	Ji, H., Fu, Q., Jiang, D.W., Shao, L. and Chen, L.Q. (2012) Physiological and Morphological Changes of Acer fabri Seedlings under Drought Stress. Acta Agriculturae Jiangxi, 24, 1-4.
[13]	Poutari, S., Abbaspour, H., Sinaki, J.M. and Makarian, H. (2013) Studying Activities of Some Antioxidant Enzymes in Two Castor Grade under Drought Stress and Nitrogen Fertilizer. Bulletin of Environment, Pharmacology and Life Science, 2, 106-111.
[14]	Zhang, L.X., Gao, M., Hu, J.J., Zhang, X.F., Wang, K. and Ashraf, M. (2012) Modulation Role of Abscisic Acid (ABA) on Growth, Water Relations and Glycinebetaine Metabolism in Two Maize (Zea mays L.) Cultivars under Drought Stress. International Journal of Molecular Sciences, 13, 3189-3202. http://dx.doi.org/10.3390/ijms13033189
[15]	Larhera, F.R., Gagneulb, D., Niogreta, M-F., Emeryc, N., Monniera, C., Maerskalcka, V., Quilliena, G., Leforta, C., Plasmana, M., Boucharda, F. and Bouchereaua, A. (2013) Potentiation of Proline Accumulation in Oilseed Rape Leaf Discs Exogenously Supplied with Combinations of PEG and Cryoprotective Agents Is Associated with Overproduction of ABA. Environmental and Experimental Botany, 87, 167-178.
[16]	Ye, N.H., Zhu, G.H., Liu, Y.G., Li, Y.X. and Zhang, J.H. (2011) ABA Controls H2O2 Accumulation through the Induction of OsCATB in Rice Leaves under Water Stress. Plant and Cell Physiology, 52, 689-698. http://dx.doi.org/10.1093/pcp/pcr028
[17]	Zhang, W., Tian, Z.G., Pan, X.J., Zhao, X.M. and Wang, F. (2013) Oxidative Stress and Non-Enzymatic Antioxidants in Leaves of Three Edible Canna Cultivars under Drought Stress-Hort. Horticulture, Environment, and Biotechnology, 54, 1-8. http://dx.doi.org/10.1007/s13580-013-0070-6
[18]	Ding, X.P., Li, X.K. and Xiong, L.Z. (2013) Insight into Differential Responses of Upland and Paddy Rice to Drought Stress by Comparative Expression Profiling Analysis. International Journal of Molecular Sciences, 14, 5214-5238.