Biomimetically Synthesized Aqueous Ferrofluids Having Antibacterial and Anticancer Properties

Lubna Sheikh^1,2, Richa Vohra³, Anita Kamra Verma³, Suprabha Nayar^1*

¹CSIR-National Metallurgical Laboratory, Jamshedpur, India.
²CSIR-Indian Institute of Chemical Biology, Kolkata, India.
³Nanobiotech Lab, Kirori Mal College, University of Delhi, Delhi, India.

DOI: 10.4236/msa.2015.63029                

Abstract

Synthesis of functional iron oxide nanoparticles, well dispersed in aqueous fluids still remains a challenge as its stability requires a delicate balance between electrostatic and magnetic interactions. Templated synthesis using biomolecules is useful because the biomolecules have their unique arrangement in aqueous systems that enhance stability, commonly called “biomimetic synthesis”. We have developed a one-pot in-situ, low energy process for the synthesis of highly monodispersed, Collagen Functionalized Ferrofluids (CFF) as a templating agent in an aqueous medium. The nanoparticles so obtained were characterized by X-ray diffraction (XRD), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR). The antibacterial activity in terms of minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and growth inhibition has been assessed against gram positive, Staphylococcus aureus, ATCC 13709 (native strain) and in Escherichia coli, DH5α gram negative bacteria. The cytotoxicity of the CFFs on cancer cell lines human embryonic kidney (HEK), breast adenocarcinoma (MCF-7) and Ehrlich ascitic carcinoma (EAC) have also been investigated. CFFs indicated variable MIC and MBC values against S. aureus and E. coli being minimum for 1.5% CFF (MIC:23.43 μg/ml and 93.75 μg/ml and MBC: 46.87 μg/ml and 187.5 μg/ml). The observed cytotoxicity in mammalian cells indicated the susceptibility of MCF-7 breast cancer cells when compared to HEK cells.

Keywords

Collagen Functionalized Ferrofluids (CFFs), Antibacterial, Biomimetics, Cytotoxicity, Anti-Cancer Activity

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Vijay, K., Varadan, L.C. and Xie, J.N. (2008) Nanomedicine: Design and Applications of Magnetic Nanomaterials, Nanosensors and Nanosystems. Wiley, Hoboken.
[2]	Arruebo, M., Fernández-Pacheco, R., Ibarra, M.R. and Santamaría, J. (2007) Magnetic Nanoparticles for Drug Deli- very. Nanotoday, 2, 22-32. http://dx.doi.org/10.1016/S1748-0132(07)70084-1
[3]	Kumar C.S.S.R. and Mohammad, F. (2011) Magnetic Nanomaterials for Hyperthermia-Based Therapy and Controlled Drug Delivery. Advanced Drug Delivery Reviews, 63, 789-808. http://dx.doi.org/10.1016/j.addr.2011.03.008
[4]	Gubin, S.P. (2009) Magnetic Nanoparticles. Wiley-VCH, Weinheim. http://dx.doi.org/10.1002/9783527627561
[5]	Chen, S., Feng, J., Guo, X., Hong, J. and Ding, W. (2005) One-Step Wet Chemistry for Preparation of Magnetite Nano- rods. Materials Letters, 59, 985-988. http://dx.doi.org/10.1016/j.matlet.2004.11.043
[6]	Nidhin, M., Indumathy, R., Sreeram, K.J. and Nair, B.U. (2008) Synthesis of Iron Oxide Nanoparticles of Narrow Size Distribution on Polysaccharide Templates. Bulletin of Materials Science, 31, 93-96. http://dx.doi.org/10.1007/s12034-008-0016-2
[7]	Li, G.Y., Jiang, Y., Huang, K., Ding, P. and Chen, J. (2008) Preparation and Properties of MagneticFe3O4-Chitosan Nanoparticles. Journal of Alloys and Compounds, 466, 451-456. http://dx.doi.org/10.1016/j.jallcom.2007.11.100
[8]	Hong, R.Y., Feng, B., Chen, L.L., Liu, G.H., Li, H.Z., Zheng, Y. and We, D.G. (2008) Synthesis, Characterization and MRI Application of Dextran-Coated Fe3O4 Magnetic Nanoparticles. Biochemical Engineering Journal, 42, 290-300. http://dx.doi.org/10.1016/j.bej.2008.07.009
[9]	Mukhopadhyay, A., Joshi, N., Chattopadhyay, K. and De, G.A. (2012) Facile Synthesis of PEG-Coated Magnetite (Fe3O4) Nanoparticles and their Prevention of the Reduction of Cytochrome C. ACS Applied Materials & Interfaces, 4, 142-149. http://dx.doi.org/10.1021/am201166m
[10]	Yiu, H.H., McBain, S.C., Lethbridge, Z.A., Lees, M.R. and Dobson, J. (2010) Preparation and Characterization of Polyethylenimine-Coated Fe3O4-MCM-48 Nanocomposite Particles as a Novel Agent for Magnet-Assisted Transfection. Journal of Biomedical Materials Research Part A, 92, 386-392. http://dx.doi.org/10.1002/jbm.a.32363
[11]	Fauconnier, N., Pons, J., Roger, J. and Bee, A. (1997) Thiolation of Maghemite Nanoparticles by Dimercaptosuccinic Acid. Journal of Colloid and Interface Science, 194, 427-433. http://dx.doi.org/10.1006/jcis.1997.5125
[12]	Wang, Y., Wong, J.F., Teng, X., Lin, X.Z. and Yang, H. (2003) Pulling Nanoparticles into Water: Phase Transfer of Oleic Acid Stabilized Monodisperse Nanoparticles into Aqueous Solutions of α-Cyclodextrin. Nano Letters, 3, 1555- 1559. http://dx.doi.org/10.1021/nl034731j
[13]	Park, J.W., Bae, K.H., Kim, C. and Park, T.G. (2011) Clustered Magnetite Nanocrystals Cross-Linked with PEI for Efficient siRNA Delivery. Biomacromolecules, 12, 457-465. http://dx.doi.org/10.1021/bm101244j
[14]	Soderholm, K.J.M. and Shang, S.W. (1993) Molecular Orientation of Silane at the Surface of Colloidal Silica. Journal of Dental Research, 72, 1050-1054. http://dx.doi.org/10.1177/00220345930720061001
[15]	Zhang, Y., Kohler, N. and Zhang, M. (2002) Surface Modification of Superparamagnetic Magnetite Nanoparticles and Their Intracellular Uptake. Biomaterials, 23, 1553-1561. http://dx.doi.org/10.1016/S0142-9612(01)00267-8
[16]	Bohren, C.F. and Huffman, D.R. (1983) Absorption and Scattering of Light by Small Particles. John Wiley and Sons, New York, 483-489.
[17]	Cummins, H.Z. and Pike, E.R. (1974) Photon Correlation and Light Beating Spectroscopy. Plenum, New York.
[18]	Berne, B.J. and Pecora, R. (1976) Dynamic Light Scattering. Wiley, New York.
[19]	Ip, M., Lui, S.L., Poon, V.K., Lung, I. and Burd, A. (2006) Antimicrobial Activities of Silver Dressings: An in Vitro Comparison. Journal of Medical Microbiology, 55, 59-63.
[20]	Mohanty, B., Verma, A.K., Claesson, P. and Bohidar, H.B. (2007) Physical and Anti-Microbial Characteristics of Carbon Nanoparticles Prepared from Lamp Soot. Nanotechnology, 18, Article ID: 445102.
[21]	Tyagi, A., Agarwal, S., Leekha, A. and Verma, A.K. (2014) Effect of Mass and Aspect Heterogeneity of Chitosan Nanoparticles on Bactericidal Activity. International Journal of Advanced Research, 2, 357-367.
[22]	Verma, A.K., Sachin, K., Saxena, A. and Bohodar, H.B. (2005) Release Kinetics from Bio Polymeric Nanoparticles Encapsulating Protein Synthesis Inhibitor—Cycloheximide, for Possible Therapeutic Applications. Current Pharmaceutical Biotechnology, 6, 121-130. http://dx.doi.org/10.2174/1389201053642349
[23]	Mahmoudi, M., Hosseinkhani, H., Hosseinkhani, M., Boutry, S., Simchi, A., Shane Journeay, W.S., Subramani, K. and Laurent, S. (2011) Magnetic Resonance Imaging Tracking of Stem Cells in Vivo Using Iron Oxide Nanoparticles as a Tool for the Advancement of Clinical Regenerative Medicine. Chemical Reviews, 111, 253-280. http://dx.doi.org/10.1021/cr1001832
[24]	Gupta, A.K. and Gupta, M. (2005) Synthesis and Surface Engineering of Iron Oxide Nanoparticles for Biomedical Ap- plications. Biomaterials, 26, 3995-4021. http://dx.doi.org/10.1016/j.biomaterials.2004.10.012
[25]	Beets-Tan, R.G.H., Van Engelshoven, J.M.A. and Greve, J.W.M. (1998) Hepatic Adenoma and Focal Nodular Hyperplasia: MR Findings with Superparamagnetic Iron Oxide-Enhanced MRI. Clinical Imaging, 22, 211-215. http://dx.doi.org/10.1016/S0899-7071(97)00117-4
[26]	Mahmoudi, M., Simchi, A., Imani, M., Milani, A.S. and Stroeve, P. (2008) Optimal Design and Characterization of Superparamagnetic Iron Oxide Nanoparticles Coated with Polyvinyl Alcohol for Targeted Delivery and Imaging. Journal of Physical Chemistry B, 112, 14470-14481. http://dx.doi.org/10.1021/jp803016n
[27]	Boyer, C., Whittaker, M.R., Bulmus, V., Liu, J. and Davis, T.P. (2010) The Design and Utility of Polymer-Stabilized Iron-Oxide Nanoparticles for Nanomedicine Applications. NPG Asia Materials, 2, 23-30. http://dx.doi.org/10.1038/asiamat.2010.6
[28]	Hong, R.Y., Feng, B., Chen, L.L., Liu, G.H., Li, H.Z., Zheng, Y. and Wei, D.G. (2008) Synthesis, Characterization and MRI Application of Dextran-Coated Fe3O4 Magnetic Nanoparticles. Biochemical Engineering Journal, 42, 290-300. http://dx.doi.org/10.1016/j.bej.2008.07.009
[29]	Saboktakin, M.R., Tabatabaie, R.M., Maharramov, A. and Ramazanov, M.A. (2010) A Synthetic Macromolecule as MRI Detectable Drug Carriers: Aminodextran-Coated Iron Oxide Nanoparticles. Carbohydrate Polymers, 80, 695-698. http://dx.doi.org/10.1016/j.carbpol.2009.11.051
[30]	Corot, C., Robert, P., Idee, J.M. and Port, M. (2006) Recent Advances in Iron Oxide Nanocrystal Technology for Medical Imaging. Advanced Drug Delivery Reviews, 58, 1471-504. http://dx.doi.org/10.1016/j.addr.2006.09.013
[31]	Mahmoudi, M., Laurent, S., Shokrgozar, M.A. and Hosseinkhani, M. (2011) Toxicity Evaluations of Superparamagnetic Iron Oxide Nanoparticles: Cell “Vision” versus Physicochemical Properties of Nanoparticles. ACS Nano, 5, 7263- 7276. http://dx.doi.org/10.1021/nn2021088
[32]	Tartaj, P., Morales, M.D.P., Veintemillas-Verdaguer, S., Gonzalez-Carreno, T. and Serna, C.J. (2003) The Preparation of Magnetic Nanoparticles for Applications in Biomedicine. Journal of Physics D: Applied Physics, 36, 182-197. http://dx.doi.org/10.1088/0022-3727/36/13/202
[33]	Sheikh, L., Mahto, N. and Nayar, S. (2015) In Situ Synthesis of Hydroxyapatite Nanocomposites Using Iron Oxide Nanofluids at Ambient Conditions. Journal of Materials Science: Materials in Medicine, 26, 5393.