Direct Shoot Regeneration from Callus of Melicope lunu-ankenda

Ab. Zuraida Rahman¹, Ayu Nazreena Othman¹, Fatin Liyana Izzati Kamaruddin¹, Aziz Bin Ahmad^2,3

¹Biotechnology Research Centre, MARDI Headquarters, Persiaran MARDI-UPM, Serdang, Malaysia.
²School of Science and Food Technology, Universiti Malaysia Terengganu, Kuala Nerus, Malaysia.
³Centre for Fundamental and Liberal Education, Universiti Malaysia Terengganu, Kuala Nerus, Malaysia.

DOI: 10.4236/ns.2015.72009                

Abstract

Melicope lunu-ankenda is commonly used in traditional medicine. The conventional propagation method for this species is inefficient due to low propagation rate and its lengthy period to maturity. In addition, insufficient planting materials often pose a problem for the plantation sector. The tissue culture technique is best alternative to overcome the problems. The callus induction and direct shoot regeneration protocols for M. lunu-ankenda were established. Callus was successfully initiated from leaves explants cultured in MS medium added with 2,4-D at concentrations 0.5 to 5.0 mg/L singly or in combination with NAA at concentrations 1.0 to 10 mg/L. Shoot was regenerated from callus in phytohormone-free medium, BAP at concentrations 0.5 - 5.0 mg/L singly or in combination of BAP with NAA or 2,4-D at concentration 0.5 and 1.0 mg/L, respectively. BAP at 1.0 mg/L induced the highest shoot regeneration rate (80%) and number of plantlet per calli. The established methods might be used for production of phytochemicals and plantlets in large scale.

Keywords

Melicope lunu-ankenda, Regeneration, Plant Growth Regulator, In Vitro

Conflicts of Interest

The authors declare no conflicts of interest.

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