Humoral Immune Response of Immunized Sows with Recombinant Proteins of Enterotoxigenic Escherichia coli

Daniele Araujo Pereira; Caio Abércioda Silva; Mario Augusto Ono; Odilon Vidotto; Marilda Carlos Vidotto

doi:10.4236/wjv.2015.51008

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World Journal of Vaccines > Vol.5 No.1, February 2015

Humoral Immune Response of Immunized Sows with Recombinant Proteins of Enterotoxigenic Escherichia coli ()

Daniele Araujo Pereira¹, Caio Abércioda Silva², Mario Augusto Ono³, Odilon Vidotto¹, Marilda Carlos Vidotto^1*

¹Depto de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, Brazil.
²Depto de Zootecnia, Universidade Estadual de Londrina, Londrina, Brazil.
³Depto Ciências Patológicas, Universidade Estadual de Londrina, Londrina, Brazil.

DOI: 10.4236/wjv.2015.51008 PDF HTML XML 6,306 Downloads 6,875 Views Citations

Abstract

Enteric disorders in pigs are related to the fimbriae F4 (K88), F5 (K99), F6 (987P), F41 and F18 of enterotoxigenic Escherichia coli (ETEC). Immunization of sows with adhesins is important to stimulate the production of antibodies and the consequent transfer of these to the piglets via colostrum to prevent diarrhea during the neonate period and after weaning. The objective of this study was to evaluate the immune response of the sows immunized with recombinant ETEC proteins (F4, F5, F6, F18 and F41). The immune response of the sows immunized with the recombinant proteins was compared with a commercial vaccine containing ETEC bacterins. The study was performed on a commercial farm and included nine pregnant sows divided into three groups: G1 was vaccinated with recombinant proteins (n = 3); G2 was vaccinated with the commercial vaccine (n = 3); and G3 was vaccinated with sterile buffered saline (PBS) (n = 3). All the sows were fed a balanced diet without antibiotics and water ad libitum. The recombinant fimbriae stimulated the specific humoral immune response of the immunized sows. There was a statistically significant increase in the levels of antibodies to the fimbriae F4 (K88), F5 (K99), F6 (987P) and F18 in the sows vaccinated with the recombinant proteins compared with the control group. The colostrum IgG titers for all fimbriae in all the immunized sows were significantly increased compared to the control group. Additionally, all the piglets exhibited significantly increased antibody levels relative to all fimbriae when compared with those in the unimmunized control group, demonstrating successful antibody transfer via colostrum of the sows to the piglets.

Keywords

Humoral Immune Response, ETEC, Recombinant Fimbriae F4, F5, F6, F18, F41

Share and Cite:

Pereira, D. , Silva, C. , Ono, M. , Vidotto, O. and Vidotto, M. (2015) Humoral Immune Response of Immunized Sows with Recombinant Proteins of Enterotoxigenic Escherichia coli. World Journal of Vaccines, 5, 60-68. doi: 10.4236/wjv.2015.51008.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Nagy, B. and Fekete, P.Z. (2005) Enterotoxigenic Escherichia coli in Veterinary Medicine. International Journal of Medical Microbiology, 295, 443-454. http://dx.doi.org/10.1016/j.ijmm.2005.07.003
[2]	Almeida, F.S., Rigobelo, E.C., Marin, J.M., Maluta, R.P. and ávila, F.A. (2007) Diarreia suína: Estudo da etiologia, virulência e resistência a antimicrobianos de agentes isolados em leitões na região de Ribeirão Preto-SP, Brasil. Brasil. Archives Veterinária, Jaboticabal, Vol. 23, 151-157.
[3]	Mores, N. and Moreno, A.M. (2007) Neonatal Colibacillosis. In: Sobestiansky, J. and Barcello, D.E.S.N., Eds., Swine Diseases, 2nd Edition, Goiania, Canon Editorial, 110-114.
[4]	Gyles, C.L. (1994) Escherichia coli Enterotoxins. In: Gyles, C.L., Ed., Escherichia coli in Domestic Animals and Humans, CAB International, Wallingford, Oxon, UK, 337.
[5]	Fairbrother, J.M., Nadeau, E. and Gyles, C.L. (2005) E. coli in Postweaning Diarrhea in Pigs: An Update on Bacterial Types, Pathogenesis, and Prevention Strategies. Animal Health Research Review, 6, 17-39. http://dx.doi.org/10.1079/AHR2005105
[6]	Wilson, R.A. and Francis, D.H. (1986) Fimbriae and Enterotoxins Associated with Escherichia coli Serogroups Isolated from Pigs with Colibacillosis. American Journal of Veterinary Research, 47, 213-217.
[7]	Rippinger, P., Bertschinger. H.U., Imberechts, H., Nagy, B., Sorg, I., Stamm, M., Wild, P. and Wittig, W. (1995) Designations F18ab and F18ac for the Related Fimbrial Types F107, 2134P and 8813 of Escherichia coli Isolated from Porcine Postweaning Diarrhoea and from Oedema Disease. Veterinary Microbiology, 45, 281-295. http://dx.doi.org/10.1016/0378-1135(94)00141-I
[8]	Melkebeek, V., Goddeeris, B.M. and Cox, E. (2013) ETEC Vaccination in Pigs. Veterinary Immunology and Immunopathology, 152, 37-42. http://dx.doi.org/10.1016/j.vetimm.2012.09.024
[9]	Moredo, F.A., Cappuccio, J.A., Insarralde, L., Perfumo, C.J., Quiroga, M.A. and Leotta, G.A. (2012) Genotypic Characterization of Toxigenic Escherichia coli Isolated from Pigs with Postweaning Diarrhea (PWD) and Edema Disease (ED). Revista Argentina de Microbiologia, 44, 85-88.
[10]	Liu, W.X., Yuan, C.W., Meng, X.Q., Du, Y.C., Gao, R.Z., Tang, J. and Shi, D.F. (2014) Frequency of Virulence Factors in Escherichia coli Isolated from Suckling Pigs with Diarrhoea in China. The Veterinary Journal, 199, 286-289. http://dx.doi.org/10.1016/j.tvjl.2013.11.019
[11]	Vidotto, M.C., Lima, B.C.S., Fritzen, J.T.T., Freitas, J.C.D., Venancio, E.J. and Ono, M.A. (2009) Prevalence of Virulence Genes in Escherichia coli Isolated from Piglets with Diarrhea in the Parana, South Brazil. Brazilian Journal of Microbiology, 44, 515-517. http://dx.doi.org/10.1590/S1517-83822013000200030
[12]	Deacon, S.E., Roach, P.C.J., Postis, V.L.G. , Wright, G.S.A., Xia, X., Phillips, S.E.V., Knox, J.P., Henderson, P.J.F., Mcpherson, M.J. and Baldwin, S.A. (2008) Reliable Scale-Up of Membrane Protein Over-Expression by Bacterial Auto-Induction: From Microwell Plates to Pilot Scale Fermentations. Molecular Membrane Biology, 25, 588-598. http://dx.doi.org/10.1080/09687680802511774
[13]	Haesebrouck, F., Pasmans, F., Chiers, K., Maes, D., Ducatelle, R. and Decostere, A. (2004) Efficacy of Vaccines against Bacterial Diseases in Swine: What Can We Expect. Veterinary Microbiology, 100, 255-268. http://dx.doi.org/10.1016/j.vetmic.2004.03.002
[14]	Donovan, R.S., Robinson, C.W. and Glick, B.R. (1996) Review: Optimizing Inducer and Culture Conditions for Expression of Foreign Proteins under the Control of the lac Promoter. Journal Industry Microbiological, 16, 145-154. http://dx.doi.org/10.1007/BF01569997
[15]	Osek, J., Truszcyski, M., Tarasiuk, K. and Pejsak, Z. (1995) Evaluation of Different Vaccines to Control of Pig Colibacillosis under Large-Scale Farm Conditions. Comparative Immunology, Microbiology and Infectious Diseases, 18, l-8. http://dx.doi.org/10.1016/0147-9571(94)00016-N
[16]	Ruan, X., Liu, D., Casey, T.A. and Zhang, W. (2011) The Tripartite Fusion, FaeG-FedF-LT192A2:B, of Enterotoxigenic Escherichia coli (ETEC) Elicits Antibodies That Neutralize Cholera Toxin, Inhibit Adherence of K88 (F4) and F18 Fimbriae, and Protect Pigs against K88ac/Heat-Labile Toxin Infection. Clinical Vaccine Immunology, 18, 1593-1599. http://dx.doi.org/10.1128/CVI.05120-11
[17]	Riising, H.J., Murmans, M. and Witvliet, M. (2005) Protection against Neonatal Escherichia coli Diarrhea in Pigs by Vaccination of Statements with a New Vaccine That Contains Purified against Enterotoxigenic E. coli Virulence Factors F4ac, F4ab, F5 and F6 Fimbrial Antigens and Heat-Labile E. coli Enterotoxin (LT). Journal of Veterinary Medicine Series B, 52, 296-300. http://dx.doi.org/10.1111/j.1439-0450.2005.00857.x
[18]	Hur, J. and Lee, J.H. (2012) Comparative Evaluation of the Vaccine Candidate Expressing against Enterotoxigenic Escherichia coli (ETEC) Adhesions Is Colibacillosis with a Commercial Vaccine Using a Pig Model. Vaccine, 30, 3829-3833. http://dx.doi.org/10.1016/j.vaccine.2012.03.087
[19]	Morgan, R.L., Isaacson, R.E., Moon, H.W., Brinton, C.C. and To, C.C. (1978) Immunization of Suckling Pigs against Enterotoxigenic Escherichia coli-Induced Diarrheal Disease by Vaccinating Dams with Purified 987 or K99 pili: Protection Correlates with Pilus Homology of Vaccine and Challenge. Infection and Immunity, 33, 771-777.