Evaluation of the Safety of Three Phenolic Compounds from Dipteryx alata Vogel with Antiophidian Potential
Edson Hideaki Yoshida1, Miriéle Cristina Ferraz1, Natália Tribuiani1, Renata Vasques da Silva Tavares1, José Carlos Cogo2, Márcio Galdino dos Santos3, Luiz Madaleno Franco4, Cháriston André Dal-Belo5, Rone A. De Grandis6, Flávia Aparecida Resende6, Eliana Aparecida Varanda6, Pilar Puebla7, Arturo San-Feliciano7, Francisco Carlos Groppo8, Yoko Oshima-Franco1*
1Post-Graduate Program in Pharmaceutical Sciences, University of Sorocaba, Sorocaba, Brazil.
2Serpentarium of the University of Vale do Paraíba, São José dos Campos, Brazil.
3Post-Graduate Program in Environmental Sciences, Tocantins Federal University, Palmas, Brazil.
4Methodist University of Piracicaba, Piracicaba, Brazil.
5Laboratory of Neurobiology and Toxinology, Federal University of Pampa, São Gabriel, Brazil.
6Department of Biological Sciences, São Paulo State University, Araraquara, Brazil.
7Department of Pharmaceutical Chemistry, Salamanca University, Salamanca, Spain.
8Department of Physiological Sciences, University of Campinas, Piracicaba, Brazil.
DOI: 10.4236/cm.2015.61001   PDF   HTML   XML   4,242 Downloads   4,936 Views   Citations


Phenolic compounds from Dipteryx alata Vogel were assayed against the in vitro neurotoxic effect induced by Bothrops jararacussu (Bjssu) venom. Mutagenicity was assessed by the Ames test using Salmonella typhimurium strains TA98, TA97a, TA100, and TA102, in experiments with and without metabolic activation. Anti-bothropic activity was obtained by using mouse phrenic nerve-diaphragm (PND) preparation and myographic technique. Control experiments with physiological Tyrode solution were used for keeping the PND preparations alive (n = 4). Concentrations of phe-nolic compounds were as follow: protocatechuic and vanillic acids (200 μg/mL, n = 4), vanillin (50 μg/mL, n = 4). These compounds were used alone or pre-incubated with the venom (40 μg/mL), 30 min prior the addition to the organ bath (n = 4). Phenolic compounds significantly inhibited the neuromuscular blockade of Bjssu in the following order of potency: vanillic acid > protocatechuic = vanillin. Vanillic acid added 10 min after the Bjssu venom was also able to avoid the venomblockade evolution. The mutagenicity assay indicated that all phytochemicals were unable to in-crease the number of revertants, demonstrating the absence of mutagenic activity. This study demonstrated both the safety and therapeutical potential of the three phenolic compounds as novel complementary anti-bothropic agents.

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Yoshida, E. , Ferraz, M. , Tribuiani, N. , Silva Tavares, R. , Cogo, J. , dos Santos, M. , Franco, L. , Dal-Belo, C. , De Grandis, R. , Resende, F. , Varanda, E. , Puebla, P. , San-Feliciano, A. , Groppo, F. and Oshima-Franco, Y. (2015) Evaluation of the Safety of Three Phenolic Compounds from Dipteryx alata Vogel with Antiophidian Potential. Chinese Medicine, 6, 1-12. doi: 10.4236/cm.2015.61001.

Conflicts of Interest

The authors declare no conflicts of interest.


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