Cytotoxic Activity of Stem Bark Extracts from Canarium odontophyllum Miq (Dabai) against Human Colorectal Carcinoma HCT 116 Cell Line

Dayang Fredalina Basri¹, Muhammad Arifuddin Al Rasyid Mohd¹, Chan Kok Meng¹, Elda Surhaida Latif¹, Fahrul Zaman Huyop²

¹School of Diagnostic & Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
²Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Johor, Malaysia.

DOI: 10.4236/ajps.2014.526411                

Abstract

The preliminary study is aimed at evaluating the cytotoxic activity of crude extracts from the stem bark of Canarium odontophyllum against colorectal carcinoma cell HCT 116. Result of the extraction yield showed that distilled water produced the highest yield (10.58%) followed by methanol (6.55%) whereas acetone extracted the lowest yield (4.58%) from C. odontophyllum stem bark. Phytochemical screening test revealed the presence of terpenoid, flavonoid, tannin, saponin and phenolic compound in all the stem bark extracts of C. odontophyllum. Acetone extract of C. odontophyllum stem bark showed significantly higher (p < 0.05) cytotoxic effect with IC50 of 0.062 ± 1.04 mg/ml compared to methanol extract with IC50 0.122 ± 4.92 mg/ml. No cytotoxic effect was observed by the aqueous extract despite its high extraction yield. In conclusion, acetone and methanol extracts from C. odontophyllum stem bark exhibited promising cytotoxic activity against human colorectal cancer cell line HCT 116 and paves way for further investigation in view of its mode of cancer cell death.

Keywords

Canarium odontophyllum, Stem Bark, Burseraceae, HCT 116, Cytotoxic Activity, Colorectal Carcinoma

Conflicts of Interest

The authors declare no conflicts of interest.

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