The Conditions of Ovary Storage Affect the Quality of Porcine Oocytes

Matías Nicolás Tellado, Gabriel Martín Alvarez, Gabriel Carlos Dalvit, Pablo Daniel Cetica

Area of Biochemistry, INITRA (Institute of Research and Technology in Animal Reproduction), School of Veterinary Sciences, University of Buenos Aires, Buenos Aires, Argentina.

DOI: 10.4236/arsci.2014.23007                

Abstract

We studied the effect of different storage conditions of porcine ovaries (time, temperature) on the characteristics of the follicular fluid, immature oocyte quality, meiotic competence and in vitro fertilization of oocytes. Ovaries were stored for 2, 4 or 6 h at 3 different temperatures (15°C, 25°C or 35°C). As the storage time increased, pH and glucose concentration of the follicular fluid, percentage of live immature oocytes at germinal vesicle stage, oxidative activity and maturation rate decreased. At higher temperatures, pH and glucose concentration decreased, but oxidative activity and oocyte maturation rate increased. Lactate concentration and immature oocyte ROS production increased as storage time and temperature increased. The ovary storage for longer than 2 h at 25°C and 35°C resulted in low pH of the follicular fluid and high ROS level in immature oocytes. Such conditions seem to damage oocytes and impair their meiotic competence. A decrease in the oxidative activity caused by long time and/or low storage temperature may imply a decrease in oocyte vitality. In conclusion, in the porcine species, the transport of ovaries at 25°C and 35°C for 2 h are the best conditions to maintain adequate oocyte quality, meiotic competence and in vitro fertilization rates.

Keywords

Ovary Storage, Oocyte Quality, Pig

Conflicts of Interest

The authors declare no conflicts of interest.

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