Establishment and Optimization Growth of Shoot Buds-Derived Callus and Suspension Cell Cultures of Kaempferia parviflora


Callus and suspension cells culture of Kaempferia parviflora was successfully established. Meristematic shoots can be used for utilization of plant cell biosynthetic capabilities for obtaining useful products from valuable medicinal plant to meet out the pharmaceutical demand and also for studying the metabolism. The medium containing combination of 0.2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.2 mg/L napthyleneacetic acid (NAA) promoted the highest callus induction at 20%. Transferring the initiated callus on the medium with 1 mg/L 2,4-D enhanced the proliferation rate up to maximum fresh weight of 6.71 gm. Growth curve of cultured cells revealed that the cells continued to grow until 50 days of culture and showed the highest peak (fresh weight) at 40 days in all different initial weight tested ( 0.2, 0.5 and 1.0 gram). Isolated embryogenic callus was found to produce the highest in weight when suspended in liquid medium supplemented with 1 mg/L 2,4-D at 110 rpm resulted 13.5 gram fresh weight and 1080 mg dry weight.

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Zuraida, A. , Nazreena, O. , Liyana Izzati, K. and Aziz, A. (2014) Establishment and Optimization Growth of Shoot Buds-Derived Callus and Suspension Cell Cultures of Kaempferia parviflora. American Journal of Plant Sciences, 5, 2693-2699. doi: 10.4236/ajps.2014.518284.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Yenjai, C., Prasanphen, K., Daodee, S., Wongpanich, V. and Kittakoop, P. (2004) Bioactive Flavonoids from Kaempferia parviflora. Fitoterapia, 75, 89-92.
[2] Rujjanawate, C., Kanjanapothi, D., Amornlerdpison, D. and Pojanagaroon, S. (2005) Anti-Gastric Ulcer Effect of Kaempferia parviflora. Journal of Ethnopharmacology, 102, 120-122.
[3] Tewtrakul, S., Subhadhirasakul, S. and Kummee, S. (2008) Anti-Allergic Activity of Compounds from Kaempferia parviflora. Journal of Ethnopharmacology, 116, 191-193.
[4] Trisomboon, H. (2009) Kaempferia parviflora: A Thai Herbal Plant, Neither Promote Reproductive Function Nor Increase Libido via Male Hormone. Thai Journal of Physiological Sciences, 21, 83-86.
[5] Wongsinkongman, P., Mongkolchaipak, N., Chansuvanich, N., Techadumrongsin, Y. and Boonruad, T. (2003) Quality Evaluation of Crude Drugs and Volatile Oil of Krachai-Dam Rhizomes. Bulletin of the Department of Medical Sciences, 45, 1-16.
[6] Azuma, T., Tanaka, Y. and Kikuzaki, H. (2008) Phenolic Glycosides from Kaempferia parviflora. Phytochemistry, 69, 2743-2748.
[7] Sutthanut, K., Sripanidkulchai, B., Yenjai, C. and Jay, M. (2007) Simultaneous Identification and Quantitation of 11 Flavonoid Constituents in Kaempferia parviflora by Gas Chromatography. Journal of Chromatography A, 1143, 227-233.
[8] Sookkongwaree, K., Geitman, M., Roengsumran, S., Petsom, A. and Danielson, U.N. (2006) Inhibition of Viral Proteases by Zingiberaceae Extracts and Flavones Isolated from Kaempferia parviflora. Die Pharmazie, 61, 717-721.
[9] Sawasdee, P., Sabphon, C., Sitthiwongwanit, D. and Kokpol, U. (2009) Anticholinesterase Activity of 7-Methoxy-flavones Isolated from Kaempferia parviflora. Phytotherapy Research, 23, 1792-1794.
[10] Rosen, R.C. and Kostis, J.B. (2003) Overview of Phosphodiesterase 5 Inhibition in Erectile Dysfunction. American Journal of Cardiology, 92, 9-18.
[11] Park, J.E., Pyun, H.B., Woo, S.W., Jeong, J.H. and Hwang, J.K. (2014) The Protective Effect of Kaempferia parviflora Extract on UVB-Induced Skin Photoaging in Hairless Mice. Photodermatology, Photoimmunology & Photomedicine, Early View.
[12] Prathanturarug, S., Apichartbutra, T., Chuakul, W. and Saralamp, P. (2000) Mass Propagation of Kaempferia parviflora Wall ex Baker by in Vitro Regeneration. Journal of Horticultural Science and Biotechnology, 82, 179-183.
[13] Shirin, A.P.R. and Jamuna, P. (2010) Chemical Composition and Antioxidant Properties of Ginger Root (Zingiber officinale). Journal of Medicinal Plants Research, 4, 2674-2679.
[14] Mello, M.O., Amaral, A.F.C. and Melo, M. (2000) Quantificação da micropropagação de Curcuma zedoaria Roscoe. Scientia Agricola, 57, 703-707.
[15] Salvi, N.D., George, L. and Eapen, S. (2001) Plant Regeneration from Leaf Base Callus of Turmeric and Random Amplified Polymorphic DNA Analysis of Regenerated Plants. Plant Cell, Tissue and Organ Culture, 66, 113-119.
[16] Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497.
[17] Saensouk, P. (2011) Callus Induction and Plant Regeneration from Leaf Explant of Cornukaempferia aurantiflora Mood & Larsen. Pakistan Journal of Botany, 43, 2415-2418.
[18] Saensouk, P., Theerakulpisut, P., Kijwijan, B. and Bunnag, S. (2007) Effects of 2,4-D on Callus Induction from Leaf Explants of Cornukaempferia larsenii P. Gardens’ Bulletin, 59, 183-188.
[19] Ilahi, I. and Jabeen, M. (1987) Micropropagation of Zingiber officinale L. Pakistan Journal of Botany, 19, 61-65.
[20] Vincent, K.A., Hariharan, M. and Mathew, K.M. (1992) Embryogenesis and Plantlet Formation in Tissue Culture of Kaempferia galangal L.: A Medicinal Plant. Phytomorphology, 42, 253-256.
[21] Guo, Y.H. and Zhang, Z.X. (2005) Establishment and Plant Regeneration of Somatic Embryogenesis Cell Suspension Cultures of the Zingiber officinale Rosc. Scientia Horticulturae, 107, 90-96.

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