Replacement of Soybean Meal with Lupin or Chickpea Seed Meal in Diets for Fattening Iberian Pigs Promotes a Healthier Ileal Microbiota Composition

Luis A. Rubio, M. Jesús Peinado

Fisiología y Bioquímica de la Nutrición Animal (EEZ, CSIC), Granada, Spain.

DOI: 10.4236/aim.2014.49055                

Abstract

Five castrated male Iberian pigs (100 ± 2 kg b.w.) fitted with T-shaped ileal cannulas at the terminal ileum were used to determine the effects of legume feeding on intestinal microbiota composition. The diets were based on defatted soybean (Glycine max), lupin (Lupinus angustifolius) or chickpea (Cicer arietinum) seed meals and contained similar amounts of digestible energy (14.2 - 15.1 MJ·kg^-1) and protein (107 g·kg^-1). A hydrolyzed casein diet was used to determine the bacterial counts in pigs fed on a vegetable-free diet. The composition of the intestinal microbiota at the terminal ileum was analysed by q-PCR. Higher (P < 0.05) lactobacilli log₁₀ number of copies was determined in the ileal contents of pigs fed on lupin- or chickpea-based diets with respect to those fed on the soybean-based diet. Bacteroides and the Clostridium coccoides/Eubacterium rectale group log₁₀ number of copies was lower (P < 0.01) than that of soybean in the ileal contents of chickpea-fed pigs. Enterobacteria and the Escherichia/Shigella group log₁₀ number of copies was lower (P < 0.01) than that of soybean in pigs fed on diets based on lupin or chickpea. The number of copies of the different bacterial groups in animals fed on the casein-based diet was lower (P < 0.01) than that of soybean for lactobacilli and bacteroides, but was higher than that of soybean for bifidobacteria, enterobacteria and the Escherichia/Shigella group. This information suggests that lupin or chickpea feeding might induce a benefit in the microbiota composition of Iberian pigs in their final productive stages.

Keywords

Defatted Soybean, Lupin, Chickpea, Iberian Pig, Ileal Microbiota

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Rey, A.I. and Lopez-Bote, C. (2001) Effect of Dietary Copper and Vitamin E Supplementation, and Extensive Feeding with Acorn and Grass on Longissimus Muscle Composition and Susceptibility to Oxidation in Iberian Pigs. Journal of Animal Physiology and Animal Nutrition, 85, 281-292. http://dx.doi.org/10.1046/j.1439-0396.2001.00316.x
[2]	Nieto, R., Rivera, M., García, M.A. and Aguilera, J.F. (2002) Amino Acids Availability and Energy Value of Acorn in the Iberian Pig. Livestock Production Science, 77, 227-239. http://dx.doi.org/10.1016/S0301-6226(02)00040-4
[3]	Aparicio Macarro, J.B. (1992) La montanera y el cerdo Ibérico. In: El cerdo Ibérico, la naturaleza y la dehesa, Ministerio de Agricultura, Pesca y Alimentación, 169-187. [Free Land Feeding and the Iberian Pig. In: The Iberian Pig, the Environment and the woodland Prairie, Spanish Ministry of Agriculture, Fisheries and Food, 169-187.]
[4]	Spanish Administration (2007) REAL DECRETO 1469/2007 por el que se aprueba la norma de calidad para la carne, el jamón, la paleta y la cana de lomo ibéricos. BOE No. 264. [Spanish Administration (2007) Royal Decree 1469/2007 for the Approval of the Quality Law for Iberian Pig Meat, Ham and Loin. BOE No. 264.]
[5]	Gaskins, H.R. (2001) Intestinal Bacteria and Their Influence on Swine Growth. In: Lewis, A.J. and Southern, L.L., Eds., Swine Nutrition, 2nd Edition, Taylor and Francis Inc., Boca Roca, 585-608.
[6]	Gaggìa, F., Mattarelli, P. and Biavati, B. (2010) Probiotics and Prebiotics in Animal Feeding for Safe Food Production. International Journal of Food Microbiology, 141, S15-S28. http://dx.doi.org/10.1016/j.ijfoodmicro.2010.02.031
[7]	Williams, B.A., Verstegen, M.W.A. and Tamminga, S. (2001) Fermentation in the Large Intestine of Single-Stomached Animals and Its Relationship to Animal Health. Nutrition Research Reviews, 14, 207-227. http://dx.doi.org/10.1079/NRR200127
[8]	Rubio, L.A. (2005) Ileal Digestibility of Defatted Soybean, Lupin and Chickpea Meals in Cannulated Adult Iberian Pigs. I. Proteins. Journal of the Science of Food and Agriculture, 85, 1313-1321. http://dx.doi.org/10.1002/jsfa.1963
[9]	Rubio, L.A., Pedrosa, M.M., Pérez, A., Cuadrado, C., Burbano, C. and Muzquiz, M. (2005) Ileal Digestibility of Defatted Soybean, Lupin and Chickpea Meals in Cannulated Adult Iberian Pigs. II. Fatty Acids and Carbohydrates. Journal of the Science of Food and Agriculture, 85, 1322-1328. http://dx.doi.org/10.1002/jsfa.1964
[10]	Canibe, N. and Eggum, B.O. (1997) Digestibility of Dried and Toasted Peas in Pigs. 2. Ileal and Total Tract Digestibilities of Amino Acids, Protein and Other Nutrients. Animal Feed Science and Technology, 64, 311-325. http://dx.doi.org/10.1016/S0377-8401(96)01038-3
[11]	National Research Council (1998) Nutrient Requirements of Swine. 10th Revised Edition, National Academy Press, Washington DC.
[12]	Ruiz, R. and Rubio, L.A. (2009) Lyophyllization Improves the Extraction of PCR-Quality Community DNA from Pig Fecal Samples. Journal of the Science of Food and Agriculture, 89, 723-727. http://dx.doi.org/10.1002/jsfa.3465
[13]	Rubio, L.A., Peinado, M.J., Ruiz, R., Suárez-Pereira, E., Ortiz Mellet, C. and García Fernández, J.M. (2014) A Di-D-Fructose Dianhydride-Enriched Caramel Modulates Pig Fecal Microbiota Composition. Advances in Microbiology, 4, 242-251. http://dx.doi.org/10.4236/aim.2014.45031
[14]	Jezierny, D., Mosenthin, R. and Bauer, E. (2010) The Use of Grain Legumes as a Protein Source in Pig Nutrition: A Review. Animal Feed Science and Technology, 157, 111-128. http://dx.doi.org/10.1016/j.anifeedsci.2010.03.001
[15]	Rubio, L.A. (2004) Carbohydrates Digestibility and Faecal N Excretion in Rats Fed Raw and Germinated Faba Bean-(Vicia faba) and Chickpea-(Cicer arietinum) Based Diets. British Journal of Nutrition, 90, 301-309. http://dx.doi.org/10.1079/BJN2003903
[16]	Goodlad, J.S. and Mathers, J.C. (1990) Large Bowel Fermentation in Rats Given Diets Containing Raw Peas (Pisum sativum). British Journal of Nutrition, 64, 569-587. http://dx.doi.org/10.1079/BJN19900057
[17]	Schulze, H., van Leeuwen, P., Verstegen, M.W.A., Huisman, J., Souffrant, W.B. and Ahrens, F. (1994) Effect of Level of Dietary Neutral Detergent Fibre on Ileal Apparent Digestibility and Ileal Nitrogen Losses in Pigs. Journal of Animal Science, 72, 2362-2368.
[18]	Bindelle, J., Leterme, P. and Buldgen, A. (2008) Nutritional and Environmental Consequences of Dietary Fibre in Pig Nutrition: A Review. Biotechnology, Agronomy, Society and Environment, 12, 69-80.
[19]	Rubio, L.A., Spencer, R., Grant, G and Pusztai, A. (1995) The Inclusion of Lupin (Lupinus angustifolius) Seed Meal or Its Fibre Residue in the Diet Reduces Escherichia coli Counts in Both Small and Large Intestines of the Rat. Microbial Ecology in Health and Disease, 8, 101-105. http://dx.doi.org/10.3109/08910609509140086
[20]	Rubio, L.A., Brenes, A., Setien, I., Asunción, G., Durán, N. and Cutuli, M. (1998) Lactobacilli Counts in Crop, Ileum and Caecum of Growing Broiler Chickens Fed on Practical Diets Containing Whole or Dehulled Sweet Lupin (Lupinus angustifolius) Seed Meal. British Poultry Science, 39, 354-359. http://dx.doi.org/10.1080/00071669888890
[21]	Tang, X., Tian, Q., Cheng, X., Li, N. and Mao, X.-Y. (2013) Bifidobacterial Growth-Promoting Effect of Yak Milk κ-Casein Hydrolysates Produced with Different Proteases. International Journal of Food Science and Technology, 48, 1682-1687. http://dx.doi.org/10.1111/ijfs.12138
[22]	Hermes, R.G., Molist, F., Ywazaki, M., Nofrarías, M., Gomez de Segura, A., Gasa, J. and Pérez, J.F. (2009) Effect of Dietary Level of Protein and Fiber on the Productive Performance and Health Status of Piglets. Journal of Animal Science, 87, 3569-3577. http://dx.doi.org/10.2527/jas.2008-1241
[23]	Mossé, J. (1990) Nitrogen to Protein Conversion Factor for Ten Cereals and Six Legumes or Oilseeds. A Reappraisal of Its Definition and Determination. Variation According to Species and to Seed Protein Content. Journal of the Science of Food and Agriculture, 38, 18-24. http://dx.doi.org/10.1021/jf00091a004