Use of Desirability Function Method in Optimization of Regeneration and Callus Induction of Alhagi camelorum

Maryam Honari, Hossein Askari, Mahmood Khosrowchahli

Biotechnology, Department of Plant Breeding, Faculty of Agriculture and Natural Resources, Tehran Azad University, Science and Research Branch, Tehran, Iran.
Department of Biotechnology, Faculty of New Technology Engineering, Shahid Beheshti University, Tehran, Iran.
Department of Plant Breeding, Faculty of Agriculture and Natural Resources, Tehran Azad University, Science and Research Branch, Tehran, Iran.

DOI: 10.4236/ajps.2014.53036                

Abstract

In this study, the desirability function approach was used to simultaneously optimize multiple factors. The variables evaluated were combinations of growth regulators, media and organs in affecting the organogenesis of Alhagi. In the first step of this study, various experiments were carried out in order to optimize the callus induction and its growth. The highest rate of callus induction was achieved from the leaf segment on MS medium supplemented with 3 mg·l^-1 BAP + 6 mg·l^-1 NAA. Moreover, the highest rate of regeneration was recorded on MS medium supplemented with those 4 mg·l^-1 BAP using for leaf segments. The optimized condition of roots induction per shoot was observed in the 1/4 MS medium without activated charcoal and supplemented with 0.25 mg·l^-1 IAA. The well rooted plantlets were acclimatized and successfully established into soil where 70% plantlets were survived. The data indicate that media, BAP, and NAA levels may be manipulated to increase or decrease quality factors chosen.

Keywords

Desirability Functions; Multiple Factors; Organogenesis; Alhagi; Callus Induction; Optimized Condition

Conflicts of Interest

The authors declare no conflicts of interest.

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