A Transparent Polyimide Film as a Biological Cell Culture Sheet with Microstructures

Hirotaka Maenosono, Hirofumi Saito, Yasushiro Nishioka

Department of Precision Machinery, College of Science and Technology, Nihon University, Chiba, Japan.

DOI: 10.4236/jbnb.2014.51003                

Abstract

The research on stem cell cultures has attracted much attention due to the recent development of regenerative medicine. Therefore, higher functionalities for devices used for culturing cells are strongly demanded. In this study, we fabricated cell culture sheets using transparent polyimide (PI), parylene (PA), and polyetheretherketone (PEEK) to make polymer materials that had microstructures. We then cultured stromal marrow cells (OP9) on them and investigated the cell alignment within the microstructures. Hot embossing was used to fabricate the microstructures with a width and depth of 5 μm on the polymer substrates. Cultivation of the cells was confirmed on the transparent PI and PA sheets, however, it was not observed on the PEEK sheet. Slight alignment of the cells was also observed along with the microstructures.

Keywords

Transparent Polymide; Parylene; Polyetheretherketone; OP9 Cell; Hot Embossing

Conflicts of Interest

The authors declare no conflicts of interest.

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