Induction of Extracellular Lytic Enzymes by Fusarium solani

María de Guadalupe Moctezuma-Zárate, Juan Manuel Vargas-Morales, Juan F. Cárdenas-González, Víctor Manuel Martínez-Juárez, Ismael Acosta-Rodríguez

área Académica de Medicina Veterinaria y Zootecnia, Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Tulancingo de Bravo Hidalgo, México.
Laboratorio de Micología Experimental, Centro de Investigación y de Estudios de Posgrado, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, San Luis Potosí, México.

DOI: 10.4236/aim.2013.38A005                

Abstract

Fusarium solani is a necrotrophic parasitic fungus that causes wilt in some plants, causing severe economic losses in some areas of the country. The objective of this work was to analyze the induction of extracellular lytic enzymes produced by a strain of F. solani, isolated from a culture of tomato, in Villa de Arista, S.L.P. México. Polygalacturonase activity has a greater induction time at 10 days, and the xylanase has two times higher activity at 8 and 13 days of incubation at 28?C. Also, the xylanase activities A and B were very stable at 4?C. After 7 days of incubation, it has an activity of 100% and 96%, respectively, while polygalacturonase retains 61% of its initial activity. Both activities are better induced with glutamate and urea as nitrogen sources respectively, and both exhibit an initial pH optimum of 5.5. Finally, we didn’t find cellulase activity in the analyzing conditions.

Keywords

Fusarium solani; Necrotrophic; Fungus; Lytic; Enzymes

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	M. Mendoza, A. López, A. Oyervides, G. Martínez, C. de León and E. Moreno-Martínez, “Herencia Genética y Citoplásmica de la Resistencia a la Pudrición de la Mazorca del Maíz (Zea mays L.) Causada por Fusarium moniliforme Sheld,” Revista Mexicana de Fitopatología, Vol. 1, No. 3, 2003, pp. 267-271.
[2]	B. Groenewold, N. Mayek and J. L. Padilla-Ramírez, “Hongos Asociados a la Semilla de Fríjol (Phaseolus vulgaris L.) en Aguascalientes, México,” Revista Mexicana de Fitopatología, Vol. 22, 2003, pp. 375-378.
[3]	C. M. Bucio, O. A. Martínez and R. Morales, “Contaminación con Hongos en Maíz Recién Cosechado en el Estado de Guanajuato, Durante el Año 2003,” Memorias del VII Congreso Nacional de Ciencias de los Alimentos, 2004, pp. 425-431.
[4]	J. A. Carrillo, T. Montoya, S. García, J. Cruz, I. Márquez and A. Sañudo-Barajas, “Razas de Fusarium oxysporum f. sp. Lycopersici Zinder y Hansen, en Tomate (Lycopersicum esculentum Mill) en el Valle de Culiacán, Sinaloa, México,” Revista Mexicana de Fitopatología, Vol. 21, No. 3, 2003, pp 123-127.
[5]	Secretaria de Agricultura, “Ganadería y Desarrollo Rural. Artículos 45, 46 y 47 de la Ley Federal Sobre Metrología y Normalización del Agave, Cítricos (Naranja, Toronja y Mandarina),” Guanábana, Maíz, Trigo y Lana Pintada, 1999.
[6]	J. Inmaculada, O. de la Rosa, J. Navas-Cortés, R. Jiménez-Díaz and M. Tena, “Extracellular Xylanases from Two Pathogenic Races of Fusarium oxysporum f. Ciceris: Enzyme Production in Culture and Purification and Characterization of a Major Isoform as an Alkaline Endo-(1,4)xylanase of Low Molecular Weight,” Antonie van Leewenhoek, Vol. 88, No. 1, 2005, pp. 48-59.
[7]	M. I. Roncero, A. Di Pietro, M. C. Ruiz-Roldán, M. D. Huertas-González, F. García-Maceira, E. Méglecz, A. Jiménez, Z. Caracuel, R. Sancho-Zapatero, C. Hera, E. Gómez, M. Ruíz-Rubio, C. González and J. Páez, “Papel de Enzimas Líticas de la Pared Celular en la Patogenicidad de Fusarium oxysporum,” Revista Iberoamericana de Micología, Vol. 17, 2000, pp. S47-S53.
[8]	M. Posada, B. Patiño, S. Mirete, M. C. Muñoz, C. Vázquez and M. T. González-Jaen, “Comparative Analysis of Polygalacturonases in Isolates of Seven Species of Fusarium from Pinus pinea,” Mycological Research, Vol. 105, No. 1, 2001, pp. 100-104. http://dx.doi.org/10.1017/S0953756200003142
[9]	F. Haridon, S. Aimé, S. Duplessis, C. Alabouvette, Ch. Steinberg and Ch. Olivain, “Isolation of Differentially Expressed Genes during Interactions between Tomato Cells and a Protective or a Non-Protective Strain of Fusarium oxyasporum,” Physiological and Molecular Plant Pathology, Vol. 76, No. 1, 2011, pp. 9-19. http://dx.doi.org/10.1016/j.pmpp.2011.07.001
[10]	L. González-Candelas and P. E. Kolattukudy, “Isolation and Analysis of a Novel Inducible Pectate Lyase Gene from the Phytopathogenic Fungus fusarium solani f. sp. pisi (Nectria haematococca, Mating Population VI),” Journal of Bacteriology, Vol. 174, No. 20, 1992, pp. 6343-6349.
[11]	A. H. Dantzig, S. H. Zuckerman and M. M. AndonovRoland, “Isolation of a Fusarium solani Mutant Reduced in Cutinase Activity and Virulence,” Journal of Bacteriology, Vol. 168, No. 2, 1986, pp. 911-916.
[12]	M. M. Diniz Maia, M. M. Camargo de Moorais, M. A. de Morais Jr., E. H. Magalhaes Meol and J. L. de Lima Filho, “Production of Extracellular Lipase by the Phytopathogenic Fungus Fusarium solani FS1,” Revista de Microbiologia, Vol. 30, No. 4, 1999, pp. 304-309.
[13]	T. M. Wood, “The Cellulose of Fusarium solani,” Biochemical Journal, Vol. 121, 1971, pp. 353-362.
[14]	J. C. Tu, “An Imporved Mathur’s Medium for Growth, Sporulation, and Germination of Spores of Colletotrichum lindemuthianum,” Microbios, Vol. 44, No. 178, 1985, pp. 87-93.
[15]	N. Nelson, “A Photometric Adaptation of the Somogy Method for the Determination of Glucose,” Biological Chemistry, Vol. 275, No. 3, 1944, pp. 573-576.
[16]	J. M. Somogy, “Notes on Sugar Determination,” Journal of Biological Chemistry, Vol. 195, 1952, pp. 19-23.
[17]	O. H. Lowry, N. J. Rosebrough, A. L. Far and R. J. Randall, “Protein Measurement with the Folin Phenol Reagent,” Journal of Biological Chemistry, Vol. 193, 1951, pp. 265-275.
[18]	M. C. Ruiz-Roldan, M. D. Huertas-González, A. Di Pietro and M. I. G. Roncero, “Two Xylanase Genes of the Vascular Kilt Pathogen Fusarium oxysporum f. sc. Lycopersici Differentially Expressed during Infection of Tomato Plants,” Molecular General Genetics, Vol. 261, No. 3, 1999, pp. 530-536. http://dx.doi.org/10.1007/s004380050997
[19]	C. Díaz-Polanco and Y. M. Camino, “Una Nueva Forma de Fusarium solani, Patógeno del Apio (Arracacia xanthorriza) en Venezuela,” Agronomía Tropical, Vol. 26, No. 4, 1980, pp. 353-358.
[20]	M. A. Quilambaqui, “Aislamiento e Identificación de Especies de Fusarium sp Asociadas al Declinamiento del Espárrago (Asparagus officinalis L.) en Cinco Municipios de Guanajuato, México,” Fitopatología, Vol. 18, No. 1, 2001, pp. 17-22.
[21]	C. Flores, S. San Martín, L. Carrillo and N. Bejarano, “Especies de Fusarium de la Quebrada de Lozano, Jujuy, Argentina,” Revista Argentina de Microbiología, Vol. 37, No. 2, 2005, pp. 109-112.
[22]	G. E. Kikot, R. A. Hours and T. M. Alconada, “Extracellular Enzymes of Fusarium graminearum Isolates,” Brasilian Archives of Biology and Technology, Vol. 53, No. 4, 2010, pp. 779-783.
[23]	R. J. Tweddell, S. H. Jabaji-Hare and P. M. Charest, “Production of Chitinases and β-1,3-Glucanases by Stachybotrys elegans, a Mycoparasite of Rhizoctonia solani,” Applied and Environmental Microbiology, Vol. 60, No. 2, 1994, pp. 489-495.
[24]	E. Lin and D. B. Wilson, “Regulation of β-1,4-endoglucanase Synthesis in Thermomonospora fusca,” Applied and Environmental Microbiology, Vol. 53, No. 6, 1987, pp. 1352-1357.
[25]	P. Magnelli, A. M. Ramos and F. Forchiassin, “Factors Influencing Cellulase Production by Saccobolus saccoboloides,” Mycologia, Vol. 88, No. 2, 1996, pp. 249-255. http://dx.doi.org/10.2307/3760929
[26]	I. Acosta-Rodríguez, C. Piñon-Escobedo, M. G. ZavalaPáramo, E. López-Romero and H. Cano-Camacho, “Degradation of Cellulose by the Bean-Pathogenic Fungus Colletotrichum lindemuthianum. Production of Extracellular Cellulolytic Enzymes by Cellulose Induction,” Antonie van Leeuwenhoek, Vol. 87, No. 4, 2005, pp. 301-310.
[27]	M. T. Yazdi, J. R. Woodward and A. Roadford, “The Cellulose Complex of Neurospora crassa: Activity Stability and Release,” Journal of General Microbiology, Vol. 136, No. 7, 1990, pp. 1313-1319. http://dx.doi.org/10.1099/00221287-136-7-1313
[28]	C. P. Kubicek, G. Mülhlbauer, M. Klotz, E. John and E. M. Kubicek-Pranz, “The Trichoderma Cellulose Regulatory Puzzle: From the Interior Life of a Secretory Fungus,” Enzyme Microbiology and Technology, 1988, pp. 1215-1222.
[29]	C. Cano-Canchola, L. Acevedo, P. Ponce-Noyola, A. Flores-Martínez, A. Flores-Carreón and C. A. LealMorales, “Induction of Lytic Enzymes by the Interaction of Ustilago maydis with Zea mays Tissues,” Fungal Genetics and Biology, Vol. 29, No. 3, 2000, pp. 145-149. http://dx.doi.org/10.1006/fgbi.2000.1196
[30]	L. Hernández-Silva, C. Piñón-Escobedo, H. Cano Camacho, M. G. Zavala-Páramo, I. Acosta-Rodríguez and E. López-Romero, “Comparison of Fungal Growth and Production of Extracellular Pectin Lyase Activity by Pathogenic and Non-Pathogenic Races of Colletotrichum lindemuthianum Cultivated under Different Conditions,” Physiological and Molecular Plant Pathology, Vol. 70, No. 1-3, 2007, pp. 88-95. http://dx.doi.org/10.1016/j.pmpp.2007.07.005
[31]	A. R. Corrales Escobosa, R. A. Rangel Porras, V. Meza Carmen, G. A. González Hernández, J. C. Torres Guzmán, K. Wrobel, K. Wrobel, M. I. Roncero and J. F. Gutiérrez Corona, “Fusarium oxysporum Adh1 Has Dual Fermentative and Oxidative Functions and Is Involved in Fungal Virulence in Tomato Plants,” Fungal Genetics and Biology, Vol. 48, No. 9, 2011, pp. 886-895.