Yield Evaluation of a Wheat Line with Combined Resistance to Russian Wheat Aphid and Stem Rust Race “Ug99” in Kenya

F. O. Amulaka, J. N. Maling’a, R. S. Pathak, M. Cakir, R. M. S. Mulwa

Egerton University, Department of Crops, Horticulture and Soils, Egerton, Kenya.
Egerton University, Department of Crops, Horticulture and Soils, Egerton, Kenya;.
Kenya Agricultural Research Institute (KARI), Njoro, Kenya..
Murdoch University, Perth, Australia.

DOI: 10.4236/ajps.2013.47181                

Abstract

InKenya, Russian wheat aphid (RWA) and stem rust race TTKS (“Ug99”) are the most devastating pests of wheat. Severe infestations by RWA result in yield losses of up to 90% while epidemics of “Ug99”can cause up to 100% loss. The two pests combined have seriously affected farmer incomes forcing them to rely heavily on pesticides and increasing the cost of production. This study sought to evaluate a wheat line that has been developed to be resistant to both RWA and “Ug99”by pyramiding two major resistance genes. Three varieties were used in this study: “Kwale”, a Kenyan high yielding commercial variety but susceptible to both RWA and “Ug99”; “Cook”, an Australian variety carrying stem rust resistance gene Sr36 conferring immunity to “Ug99”; and “KRWA9”, a Kenyan line resistant to RWA but with poor agronomic attributes. The F₁ of the double cross (DC F₁) was obtained by crossing the F₁ of “Kwale × Cook” and the F₁ of “Kwale × KRWA9”. The DC F₁ population was subjected to sequential screening for both RWA and “Ug99”resistance. The surviving DC F₁ progenies were left to self pollinate in the field to obtain the DC F₂. The DC F₂ progenies were sequentially screened against RWA and “Ug99”to obtain a resistant population to both RWA and “Ug99”. The yield and yield components of the new resistant line were compared with the three parents. Results showed that the DC F_2:3 had higher yields than the three parents based on 1000 kernel weight, weight of kernel per spike, and the actual yield in tons/ha, indicating that the genes were successfully introgressed. It is concluded that though races with virulence for Sr36 have been reported, the gene provides immunity to race “Ug99”and can be used as a component for “Ug99”resistance breeding together with other Sr genes.

Keywords

Rusian Wheat Aphid; Stem Rust “Ug99”; Gene Pyramiding; Combined Resistance

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	O. V. Kovalev, T. J. Poprawski, A. V. Stekolshchikov, A. B. Vereshchagina and S. A. Gandrabur, “Diuraphis Aizenberg (Hom. Aphididae); Key Apterous Viviparous Females, and Review of Russian Literature on the Natural History of Diuraphis noxia (Kurdjumov, 1913),” Journal of Applied Entomology, Vol. 112, 1991, pp. 425-436. doi:10.1111/j.1439-0418.1991.tb01076.x
[2]	J. Webster, S. Amosson, L. Brooks, G. Hein, G. Johnson, D. Legg, W. Massey, P. Morrison, F. Peairs and M. Weiss, “Economic Impact of the Russian Wheat Aphid in the Western United States: 1992-1993,” Great Plains Agricultural Council Publication, 1994, p. 152.
[3]	P. H. Hewitt, “The South African Experience with the Russian Wheat Aphid, 1-3,” In: F. B. Peairs and S. D. Pilcher, Eds., Proceedings 2nd Russian Wheat Aphid Workshop, Colorado State University, Fort Collins, 1988.
[4]	M. Macharia, P. M. Muthangya and J. K. Wanjama, “Response to Seed-Dressing Aphicides in Commercial Varieties Russian Wheat Aphid (Diuraphis noxia) Damage in Kenya,” Proceedings of the 10th Regional Wheat Workshop for Eastern, Central and Southern Africa, Pretoria University, Pretoria, 1999, pp. 418-421.
[5]	J. N. Maling’a, “Studies on Russian Wheat Aphid (Diuraphis noxia Kurdjumov) with Special Emphasis to Biotypes and Host Plant Resistance in Bread Wheat (Triticum aestivum L.) in Kenya,” Ph.D. Thesis, Egerton University, Njoro, 2007.
[6]	M. Kinyua, J. Maling’a, L. Karanja and J. Wanjama, “Application of Biotechnology in Development of Bread Wheat Varieties Resistant/Tolerant to Russian Wheat Aphid (Diuraphis noxia),” Proceedings of the 7th KARI Biennial Scientific Conference, Collaborative and Participatory Research for Sustainable Improved Livelihoods, 13-17 November 2000.
[7]	J. P. Michaud and P. E. Sloderbeck, “Russian Wheat Aphid: An Introduced Pest of Small Grains in the High Plains,” Kansas State University Press, Lawrence, 2005.
[8]	N. L. V. Lapitan, “Fractionated Extracts of Russian Wheat Aphid Eliciting Defense Responses in Wheat,” Journal of Economic Entomology, Vol. 100, No. 3, 2007, pp. 990-999. doi:10.1603/0022-0493(2007)100[990:FEORWA]2.0.CO;2
[9]	J. N. Maling’a, M. G. Kinyua, A. W. Kamau, J. K. Wanjama, J. O. Awalla and R. S. Pathak, “Biotypic and Genetic Variation within Tropical Populations of Russian Wheat Aphid, Diuraphis noxia (Kurdjumov) (Homoptera: Aphididae) in Kenya,” Journal of Entomology, Vol. 4, No. 5, 2007, pp. 350-360. doi:10.3923/je.2007.350.361
[10]	R. S. Pathak, K. J. Kenduiwa, M. G. Kinyua and J. N. Maling’a, “Evidence of Duplicate Gene Loci for Resistance to Russsian Wheat Aphid (Duiraphis noxia Mordvilko) in Bread Wheat,” Proceedings of African Crop Science, Vol. 8, 2007, pp. 703-708.
[11]	K. Kenduiwa, “Genetics of Resistance to Russian Wheat Aphid (Diuraphis noxia Mordvilko) in Bread Wheat (Triticum aestivum L.),” MSc. Thesis, Egerton University, Njoro, 2009.
[12]	J. Berner, “Biochemistry of Russian Wheat Aphid Resistance in Wheat: Involvement of Lipid-Like Products,” Ph.D. Thesis, Department of Plant Sciences, Faculty of Natural and Agricultural Sciences, University of the Free State Bloemfontein, 2006.
[13]	V. L.Tolmay, “Genetic Variability for Russian Wheat Aphid, Diuraphis noxia Resistance in South African Wheat Genotypes,” Ph.D. Thesis, University of the Free State, Bloemfontein, 2006.
[14]	M. C. Walters, “Progress in Russian Wheat Aphid (Diuraphis noxia Mordw.) Research in the Republic of South Africa,” Proceedings of a Meeting of the Russian Aphid Task Team, University of the Orange Free State, Bloemfontein, 5-6 May 1982.
[15]	J. L. Kurt, “Black Stem Rust Biology and Threat to Wheat Growers, U.S. Department of Agriculture,” Report to the Central Plant Board Meeting, Lexington, 5-8 February 2001.
[16]	CIMMYT, “Sounding the Alarm on Global Stem Rust. An Assessment of Ug99 in Kenya and Ethiopia and Potential for Impact in Neighboring Regions and Beyond,” CIMMYT Circular, 29 May 2007.
[17]	R. Wanyera, M. G. Kinyua, Y. Jin and R. P. Sing, “The Spread of Stem Rust Caused by Puccinia graminis f.sp. Tritici, with Virulence on Sr31 in Wheat in Eastern Africa,” Plant Disease, Vol. 90, No. 1, 2006, p. 113. doi:10.1094/PD-90-0113A
[18]	D. Hildebrant, “Future World Wheat Crops Threatened by Ug99 Stem Rust,” Farm and Ranch Guide, 2008.
[19]	CIMMYT, “Sounding the Alarm on Global Wheat Rust,” CIMMYT, Mexico City, 2005. http://www.globalrust.org/uploads/documents/SoundingAlarmGlobalRust.pdf
[20]	R. P. Singh, D. P. Hodson, J. Huerta-Espino and M. Kinyua, “Will Stem Rust Destroy the World’s Wheat Crop? Advances in Agronomy, Vol. 98, 2008, pp. 271-309. doi:10.1016/S0065-2113(08)00205-8
[21]	R. P. Singh, D. P. Hodson, Y. Jin, J. Huerta-Espino and M. Kinyua, “Current Status, Likely Migration and Strategies to Mitigate the Threat to Wheat Production from Race Ug99 (TTKS) of Stem Rust Pathogen,” CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources, Vol. 1, No. 54, 2006, pp. 1-13. doi:10.1079/PAVSNNR20061054
[22]	J. Ellis, R. Mago, R. Kota, P. Dodds, H. McFadden, G. Lawrence, W. Spielmeyer and E. Lagudah, “Wheat Rust Resistance Research at CSIRO,” Australian Journal of Agricutural Research, Vol. 58, No. 6, 2007, pp. 607-511. doi:10.1071/AR06151
[23]	I. A. Dundas, D. C. Verlin, R. F. Park, H. S. Bariana, D. R. Anugrahwati, R. Mago and A. K. M. R. Islam, “New Sources of Rust Resistance from Alien Species: Meliorating Linked Defects and Discovery,” Australian Journal of Agricutural Research, Vol. 58, No. 6, 2007, pp. 545-549. doi:10.1071/AR07056
[24]	R. P. Singh, D. P. Hodson, J. Huerta-Espino, Y. Jin, S. Bhavani, P. Njau, S. Herrera-Foessel, P. K. Singh, S. Singh and V. Govindan, “The Emergence of Ug99 Races of Stem Rust Fungus Is a Threat to World Wheat Production,” Annual Review of Phytopathology, Vol. 49, 2011, pp. 465-681. doi:10.1146/annurev-phyto-072910-095423
[25]	R. Jaetzold and H. Schmidt, “Farm Management Handbook of Kenya, Vols. 2 B Central Kenya (Rift Valley and Central Provinces),” Ministry of Agriculture in Cooperation with GAT of GTZ, Nairobi, 2007.
[26]	R. S. Pathak, J. N. Maling’a, M. G. Kinyua, J. K. Wanjama and A. W. Kamau, “Genes for Resistance to Russian Wheat Aphid to Two New Wheat Lines,” 4th International Crop Science Congress, Maputo, 2004.
[27]	R. P. Singh, D. P. Hodson, Y. Jin, J. Huerta-Espin, M. G. Kinyua, R. Wanyera, P. Njau and R. W. Ward, “Current Status, Likely Migration and Strategies to Mitigate the Threat to Wheat,” 2006.
[28]	R. H. Painter, “Insect Resistance in Crop Plants,” Macmillan, New York, 1951.
[29]	J. Lage, B. Skonvamand and S. B. Anderson, “Resistance Categories of Synthetic Hexaploid Wheat Resistant to the Russian Wheat Aphid (Diuraphis noxia),” Euphytica, Vol. 136, No. 3, 2004, pp. 291-296. doi:10.1023/B:EUPH.0000032732.53350.93
[30]	D. L. Hartl and A. G. Clark, “Principles of Population Genetics,” 4th Edition, Macmillan, New York, 2007.