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Study of Photoinduced Interaction between Calf Thymus-DNA and Bovine Serum Albumin Protein with H2Ti3O7 Nanotubes

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DOI: 10.4236/jbnb.2012.34047    3,504 Downloads   5,594 Views   Citations


Hydrogen titanate nanotubes were synthesized by hydrothermal process using 10 M NaOH and TiO2 anatase powder. The material synthesized was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to ensure the structural and morphological characteristics. The interaction of calf thymus DNA (CT-DNA) and bovine serum albumin protein with suspended aqueous solution of titanate nanotubes was investigated using UV absorption spectroscopy and the apparent association constant was found to be, Kb= 1.68 × 104 M-1 and Kap=5.41 × 103 M-1 for DNA and BSA respectively. Addition of the titanate nano material resulted quenching of fluorescence spectra of ethidium bromide-DNA in tris HCl buffer solution and that of aqueous protein solution. The apparent binding constant (Ksv= 5.46 × 104M-1 for DNA binding and Ksv = 6.063 × 103M-1 for protein binding) was deduced from relevant fluorescence quenching data using Stern-Volmer equation.

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R. Chakraborty, S. Chatterjee, S. Sarkar and P. Chattopadhyay, "Study of Photoinduced Interaction between Calf Thymus-DNA and Bovine Serum Albumin Protein with H2Ti3O7 Nanotubes," Journal of Biomaterials and Nanobiotechnology, Vol. 3 No. 4, 2012, pp. 462-468. doi: 10.4236/jbnb.2012.34047.


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