Microbiological Quality of Chicken Sold in Accra and Determination of D10-Value of E. coli


Chicken is an excellent source of good quality protein, but it is highly susceptible to microbial contamination and often implicated in food borne disease. The microbiological quality of chicken at different retail outlets (supermarkets, local markets and farms) in Accra was investigated, and D10-values of E. coli in refrigerated and frozen retailed chicken was determined. The microbiological quality of chicken was studied by analyzing 27 chicken thigh samples collected from the retail outlets. D10-value of Escherichia coli was determined by using a linear regression model after gamma irradiation of inoculated chicken samples with doses of 0, 150, 300, 450, 600, 750 and 900 Gy. Mean total viable counts for the supermarkets, local markets and farms were 6.46, 6.91 and 6.57 log10 cfu/g respectively. Mean total coliform counts for the supermarkets, local markets and farms were 3.80, 3.46 and 3.14 log10 cfu/g respectively and the mean S. aureus counts were also 2.32, 2.28 and 2.70 log10 cfu/g respectively. There were no significant differences (p > 0.05) between the mean total viable count, total coliform counts and S. aureus count for the supermarkets, local markets and the farms. Mean counts of E. coli detected at the supermarket, local markets and farms were 1.27, 2.59 and 2.74 log10 cfu/g respectively. Salmonella spp. was detected in 2 out of the 27 samples. Fifty-two percent and 70% of samples respec-tively had total viable counts and total coliform counts within the microbial safety standards. Mean D10E. coli were 0.22 and 0.32 kGy in refrigerated and frozen chicken respectively. Presence of pathogenic bacteria in fresh chicken sold in some retail outlets in Accra was confirmed. Low D10-values of E. coli especially under refrigerated conditions suggest susceptibility to low dose irradiation and possibility of controlling spoilage and pathogenic microflora of fresh poultry.

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A. Adu-Gyamfi, W. Torgby-Tetteh and V. Appiah, "Microbiological Quality of Chicken Sold in Accra and Determination of D10-Value of E. coli," Food and Nutrition Sciences, Vol. 3 No. 5, 2012, pp. 693-698. doi: 10.4236/fns.2012.35094.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] L. Kozacinski, M. Hadaziosmanovic and N. Zdolec, “Microbiological Quality of Poultry Meat on the Croatian Market,” Veterinarski Arhiv, Vol. 76, No. 14, 2006, pp. 305-313.
[2] E. Borch and P. Arinder, “Bacteriological Safety Issues in Beef and Ready-to-Eat Meat Products, as well as Montrol Measures,” Meat Science, Vol. 62, No. 3, 2002, pp. 381-390. doi:10.1016/S0309-1740(02)00125-0
[3] M. Alvarez-Astorga, R. Capita, C. Alonso-Callega, B. Moreno and M. Del Camoni Garcia Fernaudez, “Micro-Biological Quality of Retail Chicken in By-Products in Spain,” Meat Science, Vol. 62, 2002, pp. 45-50. doi:10.1016/S0309-1740(01)00225-X
[4] G. C. Mead, “Hygiene Problems and Control Process Con-Tamination,” In: G. C. Mead, Ed., Processing of Meat, Elsevier Science Publishers Ltd, New York, 1989, pp. 183-220.
[5] International Atomic Energy Agency (IAEA), “Irradiation of Poultry Meat and Its Products,” IAEA-TECDOC-688, Vienna, 1993.
[6] P. S. Mead, L. Slutsker, V. Dietz, L. F. McCaig, J. S. Bresee, C. Shapiro, P. M. Griffin and R. V. Tauxe, “Food-Related Illness and Death in the United States,” Emerging Infectious Diseases, Vol. 5, No. 5, 1999, pp. 607-625. doi:10.3201/eid0505.990502
[7] I. Klinger, H. Welgreen and D. Basker, “Microbiological Contamination of Fresh Broiler Chicken Meat in a Market,” Refuah Veterinary, Vol. 37, 1980, pp. 97-101.
[8] I. Klinger, V. Fuchs, D. Basker, E. Malenky, G. Barkat and N. Egoz, “Microbiological Quality of Industrially Processed Frozen Broiler Chickens in Israel,” Refuah Veterinary, Vol. 38, 1981, pp. 136-148.
[9] R. Blankenship, “Reduction of Spoilage and Pathogenic Bacteria,” Broiler Industry, Vol. 24, 1986.
[10] P. R. Selvan, B. Narendra, S. Sureshkumar and V. Venkatamanujam, “Microbial Quality of Retail Meat Products Available in Chennai City,” American Journal of Food Technology, Vol. 2, No. 1, 2007, pp. 55-59. doi:10.3923/ajft.2007.55.59
[11] World Health Organisation (WHO), “Prevention and Control of Foodborne Salmonellosis through the Application of the Hazard Analysis Critical Control Point System,” Report of an International Commission on Microbiological Specification for Foods (ICMSF), WHO/CDS/ VPH/86. 65, Geneva, 1986.
[12] World Health Organisation (WHO), “Health Surveillance and Management Procedures for Food Handling Personnel,” Technical Report Series No. 785, WHO, Geneva, 1989.
[13] B. W. Sheldon and L. A. Brown, “Efficacy of Ozone as a Disinfectant for Poultry Carcasses and Chill Water,” Journal of Food Science, Vol. 51, No. 2, 1986, pp. 305309. doi:10.1111/j.1365-2621.1986.tb11116.x
[14] J. M. Frels, K. J. Samuelson, G. W. Froning and J. H. Rupnow, “Evaluation of Glucose Oxidase-Catalase Treatment to Improve the Microbiological Quality of Poultry Meat,” Poultry Science, Vol. 63, 1988, pp. 841843
[15] J. Farkas, “Irradiation for Better Foods,” Trends in Food Science and Technology, Vol. 17, No. 4, 2006, pp. 148152. doi:10.1016/j.tifs.2005.12.003
[16] International Consultative Group on Food Irradiation (ICGFI), “Enhancing Food Safety through Irradiation,” International Consultative Group on Food Irradiation, FAO/IAEA, Vienna, 1999.
[17] A. Adu-Gyamfi, J. Nketsiah-Tabiri and R. Boatin, “Determination of D10 Values of Single and Mixed Cultures of Bacteria after Gamma Irradiation,” Journal of Applied Science and Technology, Vol. 14, No. 1-2, 2009, pp. 1318. doi:10.4314/jast.v14i1-2.44317
[18] D. W. Thayer, “Sources of Variation and Uncertainty in the Estimation of Radiation D10 Values for Foodborne Pathogens,” ORACBA News, Vol. 4, No. 5, 2000.
[19] J. D. Monk, L. R. Beuchat and M. P. Doyle. “Irradiation Inactivation of Food-Borne Microorganisms,” Journal of Food Protection, Vol. 58, No. 2, 1995, pp. 197-208.
[20] J. Farkas, “Irradiation as a Method for Decontaminating Food,” International Journal of Food Microbiology, Vol. 44, No. 3, 1998, pp. 189-204. doi:10.1016/S0168-1605(98)00132-9
[21] S. D. Gamage, N. G. Faith, J. B. Luchansky, D. R. Buege, and S. C. Ingham, “Inhibition of Microbial Growth in Chub-Packed Ground Beef by Refrigeration (2?C) and Medium-Dose (2.2 to 2.4 kGy) Irradiation,” International Journal of Food Microbiology, Vol. 37, No. 2-3, 1997, pp. 175-182. doi:10.1016/S0168-1605(97)00073-1
[22] M. Cheroutre-Vialette and A. Lebert, “Growth of Listeria monocytogenes as a Function of Dynamic Environment at 10?C and Accuracy of Growth Prediction with Available Models,” Food Microbiology, Vol. 71, No. 5, 2000, pp. 83-92. doi:10.1006/fmic.1999.0290
[23] J. F. Macfaddin, “Biochemical Tests for Identification of Medical Bacteria,” Williams and Wilkins, Baltimore, 1980, pp. 173-183.
[24] C. R. Stumbo, J. R. Murphy and J. Cochran, “Nature of Death Time Curves of PA 3679 and Clostridium botulinum,” Food Technology, Vol. 4, 1950, pp. 292-302.
[25] A. Amara, M. Badoum, M. Faid and K. Bouzoubaa, “Microbial Contamination of Poultry Slaughtered in Traditional Shops in Morocco,” Microbiologique, Aliments et Nutrition, Vol. 12, 1994, pp. 323-327.
[26] B. Oumokhtar, “Bacteriological Quality of Slaughtered Animals for the Preparation of Commercialised Canned Meat at Rabat,” National Doctoral Thesis, Faculty of Sciences, University of Chouaib Doukkali, El Jadida, 2000.
[27] Y. Prachasitthisak, D. Banati and H. Ito, “Shelf Life Extension of Chicken Meat by γ-Irradiation and Microflora Changes,” Food Science and Technology International, Vol. 2, No.4, 1996, pp. 242-245.
[28] Ghana Standards, Ref. Nos GS 235:1997, GS 236:1997, Ghana Standards Board, 1997.
[29] International Commission on Microbiological Specifications for Foods (ICMSF), “Microorganisms in Foods 5, Microbiological Specifications of Food Pathogens,” In: T. A. Roberts, A. C. Baird-Parker and R. B. Tompkin, Eds., International Commission on Microbiological Specifications for Foods, Blackie Academic and Professional, London, 1996.
[30] A. Chaiba, F. F. Rhazi, A. Chahloaui, B. R. Soulaymani and M. Zerhouni, “Microbiological Quality of Poultry Meat on the Mekne’s Market (Morocco),” Internet Journal of Food Safety, Vol. 9, 2007, pp. 67-71.
[31] W. G. Eisel, R. H. Linton and P. M. Muriana, “A Survey of Microbial Levels for Incoming Raw Beef in a Processing Plant,” Food Microbiology, Vol. 14, No. 3, 1997, pp. 273-282. doi:10.1006/fmic.1996.0094
[32] B. A. Sackey, P. Mensah, E. Collison and E. S. Dawson, “Campylobacter, Salmonella, Shigella and Escherichia coli in Live and Dressed Poultry from Metropolitan Accra,” International Journal of Food Microbiology, Vol. 71, No. 1, 2001, pp. 21-28. doi:10.1016/S0168-1605(01)00595-5
[33] International Consultative Group on Food Irradiation (ICGFI), “Enhancing Food Safety through Irradiation, International Consultative Group on Food Irradiation,” FAO/IAEA, Vienna, 1999.
[34] D. W. Thayer and G. Boyd, “Elimination of Escherichia coli O157:H7 in Meats by Gamma Irradiation,” Applied Environmental Microbiology, Vol. 54, No. 4, 1993, pp. 1030-1034.
[35] D. W. Thayer, G. Boyd, J. B. Fox Jr., L. Lakritz and J. W. Hampson, “Variations in Radiation Sensitivity of Foodborne Pathogens Associated with Suspending Meat,” Journal of Food Science, Vol. 60, No. 1, 1995, pp. 63-67. doi:10.1111/j.1365-2621.1995.tb05607.x
[36] M. Patterson, “Sensitivity of Bacteria to Irradiation on Poultry Meat under Various Atmospheres,” Letters in Applied Microbiology, Vol. 7, 1998, pp. 55-58. doi:10.1111/j.1472-765X.1988.tb01251.x
[37] A. Adu-Gyamfi, J. Nketsiah-Tabiri and F. Apea Bah “Radiosensitivities of Bacterial Isolates on Minced Chicken and Poached Chicken Meal and Their Elimination Following Irradiation and Chilled Storage,” Radiation Physics and Chemistry, Vol. 77, No. 2, 2008, pp. 174178. doi:10.1016/j.radphyschem.2007.04.001
[38] D. O. Halkman and H. B. An, “Death Kinetics of E. coli O157:H7, E. coli and Natural Contaminant Coliforms in Minced during Irradiation Treatment and Storage,” Turkish Journal of. Veterinary Animal Science, Vol. 28, 2004, pp. 915-920.
[39] E. Mayer-Miebach, M. R. Stahl, U. Eschrig, L. Deniaud, D. A. E. Ehlerman and H. P. Schuchman, “Inactivation of Non-Pathogenic Strain of E. coli by Ionizing Radiation,” Food Control, Vol. 16, 2005, pp. 701-705. doi:10.1016/j.foodcont.2004.06.007
[40] M. R. Clavero, J. D. Monk, L. R. Beuchat, M. P. Doyle and P. Brakett, “Inactivation of Escherichia coli O157:H7, Salmonella, and Campylobacter jejuni in Raw Ground Beef by Gamma Irradiation,” Applied Environmental Microbiology, Vol. 60, No. 6, 1994, pp. 2068-2075.
[41] J. F. Diehl, “Biological Effects of Ionizing Radiation,” In: J. F. Diehl, Ed., Safety of Irradiated Foods, Marcel Dekker Inc., New York, 1990, pp. 95-136.

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