Formaldehyde Biosensor with Formaldehyde Dehydrogenase Adsorped on Carbon Electrode Modified with Polypyrrole and Carbon Nanotube - Engineering

ENG > Vol.4 No.10, October 2012

Volume 4, Issue 10 (October 2012)

ISSN Print: 1947-3931 ISSN Online: 1947-394X

Google-based Impact Factor: 1.09 Citations

Formaldehyde Biosensor with Formaldehyde Dehydrogenase Adsorped on Carbon Electrode Modified with Polypyrrole and Carbon Nanotube ()

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Download as PDF (Size: 289KB) PP. 135-138

DOI: 10.4236/eng.2012.410B035 4,010 Downloads 5,410 Views Citations

Author(s)

Mingwei Wang, Shuhai Jiang, Yi Chen, Xiang Chen, Li Zhao, Juankun Zhang, Jinfeng Xu

Affiliation(s)

Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin Key Laboratory of Industry Microbiology, College of Biotechnology, Tianjin University of Science&Technology, Tianjin, 300457, China.

ABSTRACT

In this study, a carbon electrode electroless polymerization of polypyrrole, which formed a layer of conductive film interface,then absorped a layer of carbon nanotube particles by the way of self-assembled, is studied. The modified electrode is used to electrostatic adherence of formaldehyde dehydrogenase, with Nafion solution capped. Moreover this paper discusses the work of electrode position, PH value and scanning speed on the influence of the electrode response, the response potential is -1.2v, the optimal PH value is 7.5, and we also review the linear range of this electrode, we discovered taht this biosensor have a good linear relationship with the concentration of 1ug/ml-360ug/ml, the Correlation coefficient is 0.9983. In addition,with the stability of this electrode tested, it can be stored 15d at4°C.

KEYWORDS

Formaldehyde Dehydrogenase; Eletrostatic Self-assembly; Biosensor

Share and Cite:

M. Wang, S. Jiang, Y. Chen, X. Chen, L. Zhao, J. Zhang and J. Xu, "Formaldehyde Biosensor with Formaldehyde Dehydrogenase Adsorped on Carbon Electrode Modified with Polypyrrole and Carbon Nanotube," Engineering, Vol. 4 No. 10B, 2012, pp. 135-138. doi: 10.4236/eng.2012.410B035.

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