Age dependent expression and distribution of nitric oxide (NO) synthase isoforms in the ovine kidney - Open Journal of Molecular and Integrative Physiology

OJMIP > Vol.3 No.2, May 2013

Open Journal of Molecular and Integrative Physiology

Volume 3, Issue 2 (May 2013)

ISSN Print: 2162-2159 ISSN Online: 2162-2167

Google-based Impact Factor: 1 Citations

Age dependent expression and distribution of nitric oxide (NO) synthase isoforms in the ovine kidney ()

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Download as PDF (Size: 2341KB) PP. 61-70

DOI: 10.4236/ojmip.2013.32010 4,266 Downloads 7,390 Views Citations

Author(s)

Devin W. Davis, Megan L. Lewis, Wei Qi, David A. Hart, Francine G. Smith

Affiliation(s)

Department of Physiology & Pharmacology, Alberta Children’s Hospital Research Institute for Child and Maternal Health, University of Calgary, Calgary, Canada.
Department of Surgery, McCaig Institute for Bone and Joint Health, University of Calgary, Calgary, Canada.

ABSTRACT

The aim of the present study was to measure intrarenal spatial and temporal localization of all three nitric oxide synthase (NOS) isoforms in the developing ovine kidney. Reverse transcriptase-polymerase chain reaction (RT-PCR), Western Blot analyses, and in situ hybridization techniques were performed for NOS I -III isoforms in renal tissue obtained from sheep aged ~24 h, one, three, six, and 12 weeks post natally (N = 3). RT-PCR performed on cortical and medullary kidney tissue revealed the presence of all three NOS isoforms from day one to 12 weeks postnatally. NOS I and NOS II mRNA levels were greater in cortex compared to medulla during the first three weeks whereas NOS III mRNA levels were predominantly transcribed within the medulla. In all NOS isoforms, there was a decrease in cortical mRNA levels after three to six weeks. Protein levels confirmed the presence of all three NOS isoforms over the first three months of postnatal life. By demonstrating NOS isoform transcripts to be more abundant in the early post natal period, these findings may provide insight into the age dependent role of NO in modulating kidney function during ontogeny.

KEYWORDS

Postnatal; Kidney; Newborn; iNOS; eNOS; nNOS; Nitric Oxide

Share and Cite:

Davis, D. , Lewis, M. , Qi, W. , Hart, D. and Smith, F. (2013) Age dependent expression and distribution of nitric oxide (NO) synthase isoforms in the ovine kidney. Open Journal of Molecular and Integrative Physiology, 3, 61-70. doi: 10.4236/ojmip.2013.32010.

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