Author(s)

Bakary Ouattara^1,2, Drissa Sérémé^1*, Moustapha Koala¹, Léon Wêndé-m’minèré Nitiéma¹, Kadidia Koïta², Emmanuel Kaboré¹, Issa Wonni³

¹Laboratoire de Protection et Défense des Cultures, Institut de l’Environnement et de Recherches Agricoles, Centre National de Recherche Scientifique et Technologique, Ouagadougou, Burkina Faso.
²Laboratoire de Biosciences, Département de Biologie et Physiologie Végétale, Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso.
³Laboratoire Central d’horticulture, Institut de l’Environnement et de Recherches Agricoles, Centre National de Recherche Scientifique et Technologique, Bobo Dioulasso, Burkina Faso.

Banana streak virus (BSV) and Sugarcane bacilliform virus (SCBV) are two badnaviruses commonly found in all banana growing areas of the world. It is a threat to the production and improvement of Musa germplasm. In Burkina Faso, the presence of badnaviruses was reported in banana producing regions. The objective of this study was to determine the prevalence of BSV and SCBV in banana production areas of Burkina Faso. A survey followed by a symptomatologic study was conducted in banana plantations in 27 localities of the nine main banana producing regions from July to October 2018 and September to December 2020. In all, 251 leaf samples were collected and analysed for BSV and SCBV infection by Indirect Antigen Coated Plate Assay-ELISA followed by amplification of the RT/RNase H region using Polymerase chain reaction with Badna FP/RP and SCBV F/R primers, respectively. A variety of symptoms were observed on almost all plant organs which were revealed due to BSV by symptomatologic study. The results of serological and molecular diagnosis revealed a high overall prevalence of BSV in 80.48% of the samples tested. BSV was distributed in seven survey regions out of nine with prevalence ranging from 10% to 100% in North, Centre, Centre West, Hauts Bassins, Cascades, Centre East and Boucle of Mouhoun regions. Very low prevalence was recorded for SCBV in Cascades and East Centre region with 4.35 and 12.5%, respectively. Species detection using specific primers to each species revealed three main species: Banana streak Obino l’ewaï virus (BSOLV), Goldfinger virus (BSGFV) and Imové virus (BSIMV) in the samples tested, respectively in the proportions of 23%, 8% and 0.8%. Co-infection between BSV species was also detected.

Banana Streak Virus, Sugarcane Bacilliform Virus, Indirect Antigen Coated Plate Assay, Polymerase Chain Reaction, Musa spp

Ouattara, B. , Sérémé, D. , Koala, M. , Nitiéma, L.W. , Koïta, K. , Kaboré, E. and Wonni, I. (2023) Prevalence and Spatial Distribution of Badnavirus in the Banana (Musa spp) Major Growing Areas in Burkina Faso. American Journal of Plant Sciences, 14, 427-447. doi: 10.4236/ajps.2023.144028.