Molecular Cloning and Tissue Distribution of Troponin C from the Japanese Pearl Oyster, Pinctada fucata - American Journal of Molecular Biology

AJMB > Vol.8 No.3, July 2018

American Journal of Molecular Biology

Volume 8, Issue 3 (July 2018)

ISSN Print: 2161-6620 ISSN Online: 2161-6663

Google-based Impact Factor: 0.47 Citations

Molecular Cloning and Tissue Distribution of Troponin C from the Japanese Pearl Oyster, Pinctada fucata ()

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DOI: 10.4236/ajmb.2018.83014 812 Downloads 1,895 Views Citations

Author(s)

Daisuke Funabara^*, Yoshinori Urakawa, Satoshi Kanoh

Affiliation(s)

Graduate School of Bioresources, Mie University, Tsu, Japan.

ABSTRACT

Troponin C (TnC) is one of the subunits of troponin. Troponin, which is activated by Ca²⁺ binding, is a thin filament-associated regulator of vertebrate striated muscle contraction. The function of TnC in vertebrates has been characterized in detail, but the role of TnC in molluscan muscles is still unclear. In this work, we investigated whether TnC plays a role in the catch contraction of molluscan smooth muscle in the bivalve Japanese pearl oyster Pinctada fucata. We determined the full-length primary structure of the TnC protein from the P. fucata adductor muscle (Pifuc-TnC), and found it is composed of 150 amino acid residues with a predicted molecular weight of 17,400. Multiple sequence alignments indicated that it had four EF-hand motifs, but only one (site IV) was predicted to have Ca²⁺-binding ability. This is analogous to characterized TnCs from other mollusks. Three-dimensional modeling of Pifuc-TnC using SWISS-MODEL indicated the presence of a short loop within the α-helix connecting the site II and III EF-hand motifs. We predicted the gene structure of Pifuc-TnC using Splign alignment of our obtained cDNA and genome sequences and elucidated that Pifuc-TnC consists of five exons, with the start and stop codons located in exon 1 and exon 5, respectively. Using quantitative real-time PCR, we determined that the Pifuc-TnC gene is predominantly expressed in adductor phasic muscle and rarely in adductor catch muscle, gill, mantle and foot. These findings suggest that TnC may not have a role in catch muscle contraction.

KEYWORDS

Adductor Muscle, Catch Contraction, EF-Hand, Troponin, Troponin C

Share and Cite:

Funabara, D. , Urakawa, Y. and Kanoh, S. (2018) Molecular Cloning and Tissue Distribution of Troponin C from the Japanese Pearl Oyster, Pinctada fucata. American Journal of Molecular Biology, 8, 166-177. doi: 10.4236/ajmb.2018.83014.

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[1]	Effect of Different Species of Prorocentrum Genus on the Japanese Oyster Crassostrea gigas Proteomic Profile
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[2]	Molecular Cloning and Tissue Distribution of Troponin I from the Japanese Pearl Oyster, Pinctada fucata
	2019

[3]	Calponin Isoform Expression in the Japanese Pearl Oyster, Pinctada fucata
	2019

[4]	Troponin T from the Japanese Pearl Oyster Pinctada fucata: Molecular Cloning, Tissue Distribution, Gene Structure, and Interaction Analysis with …
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[5]	Tropomyosin Isoform Expression in the Adductor Muscle of the Japanese Pearl Oyster, Pinctada fucata
	2018

[6]	Ca2+-Induced Conformational Change of Troponin C from the Japanese Pearl Oyster, Pinctada fucata
	2018

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