In this paper, the research status of using Pinus massoniana sawdust for the cultivation of edible fungi was analyzed and discussed. It was found that Pinus massoniana sawdust contained the material base of edible fungi with sufficient cellulose, hemicellulose and lignin and a small amount of nitrogen, phosphorus, potassium, calcium, magnesium, other mineral salts, and some vitamins for the growth of edible fungi. However, the Pinus massoniana wood contained a significant amount of terpenoids, which are detrimental to the growth of edible fungus and mycelial. The major and current detoxification methods for Pinus massoniana sawdust were analyzed, such as steaming method, distillation method, lime water immersion method, outdoor piling fermentation method, and chemical method. Their advantages and disadvantages were discussed and reviewed. Finally, the major issues in the application of Pinus massoniana sawdust as the cultivation substrate of edible fungi were analyzed, and the application prospect of Pinus massoniana sawdust as the substrate for the cultivation of edible fungi was also reviewed.