The paper focuses on biodiesel production from kusum oil using esterification reaction followed by transesterification reaction in an in-house batch reactor setup. The effects of methanol to oil ratio (M/O), catalyst amount (H₂SO₄ and methodoxide) and reaction temperature on acid value and fatty acid methyl esters (FAME) is studied. Product has been analysed using FTIR spectroscopy technique for confirmation of ester group in biodiesel. Experimental data was optimized by Taguchi analysis to conclude the optimum variable affecting the response. In both processes M/O ratio has the significant effect for biodiesel production. The obtained biodiesel properties are close to commercial diesel fuel and may be rated as an alternative to conventional diesel. The biodiesel production will enhance the maximum utilisation of forestry or agricultural products.