Author(s)

Robin Y.-Y. Chiou^1*, Po-Chang Chiu², Ju-Chun Chang¹, Yu-Jang Li², Chia-Wen Hsieh³, Jin-Yi Wu³, Shu-Mei Lin¹, Yun-Lian Lin⁴, Brian B.-C. Weng³

¹Department of Food Science, National Chiayi University, Chiayi City.
²Department of Applied Chemistry, National Chiayi University, Chiayi City.
³Department of Microbiology, Immunology and Pharmaceutical Sciences, National Chiayi University, Chiayi City.
⁴Department of Chinese Pharmaceutical Science and Chinese Medicine Resources, China Medical University, Taichung City.

Biosynthesis of peanut stilbenes, including resveratrol as the secondary metabolites, could be enhanced by subjecting the kernels to germination and wound-stress. Investigations of the bio-elicited peanut sprout powder (BPSP) addressed on characterization of the comprising stilbenes and effectiveness in longevity extension deserves intensive research. In this study, peanut kernels were subjected to germination and wound-stress in preparation of BPSP. The methanol extracts of BPSP were medium pressure liquid chromatographic (MPLC) fractionated and semi-preparative HPLC recovered and followed by instrumental identification and biological activity determinations of the isolated stilbenes. In longevity experiments, 16 female 11-mon-old BALB/c mice and both genders of 12-mon-old ICR mice were daily fed with BPSP supplemented diets at doses of 0, 0.1 and 0.5 g BPSP/kg bw for 750 and 762 days, respectively. Based on chemical characterization, enriched quantity of stilbenes in the BPSP up to ca. 1% (w/w) was detected. Two new stilbene compounds, namely, 4, 5’-dihydroxy-6’’-hydroxymethyl, 6’’-methylpyrano [2’’, 3’’: 3’, 4’] stilbene and 3, 4, 5’-trihydroxy-6’’, 6’’-dimethylpyrano [2’’, 3’’: 3’, 4’]stilbene along with 5 known stilbenes were isolated. The 7 stilbenes exhibited potent antioxidative and antiglycative activities and varied with structure-activity nature. Based on the resultant survival curves and average lifespans of both mouse models, basal diets supplemented with BPSP are effective to extend mouse longevity by a dose dependent manner. It is of merit to demonstrate that peanut kernels as a potent producer could be bio-elicited to biosynthesize a broad spectrum of bioactive stilbenes to prepare BPSP which is effective to extend mouse longevity as science-evidenced by the two long-term animal experiments.

Bio-Elicited Peanut Sprout Powder (BPSP), Wound-Stress, MPLC, Peanut Stilbenes, Longevity Extension

Chiou, R. , Chiu, P. , Chang, J. , Li, Y. , Hsieh, C. , Wu, J. , Lin, S. , Lin, Y. and Weng, B. (2017) Discovery of New Stilbene Antioxidants of the Bio-Elicited Peanut Sprout Powder (BPSP) and Longevity Extension of Mice Fed with BPSP-Supplemented Diets. Food and Nutrition Sciences, 8, 141-162. doi: 10.4236/fns.2017.81010.