Author(s)

Sung Hwa Shin¹, Eun Jeoung Lee², Sunghee Hyun³, Sang Sun Kang^1*

¹Department of Biology Education, Chungbuk National University, Cheongju, Republic of Korea.
²Department of Internal Medicine, Chungbuk National University, Cheongju, Republic of Korea.
³Department of Biomedical Laboratory Science, Eulji University, Daejeon, Republic of Korea.

The TRPV4 cation channel is expressed in a broad range of tissues and participates in the generation of a Ca²⁺ signal and/or depolarization of membrane potential. Here, human phosphoglucomutase- 1 (PGM1), an enzyme that converts glucose-6 phosphate to glucose-1 phosphate in the glycolysis pathway, as the first auxiliary protein of TRPV4 Ca²⁺ channels, is identified with yeast two hybrid system, coimmunoprecipitation, confocal microscopy, and GST pull-down assays. TRPV4 forms a complex with PGM1 through its C-terminal cytoplasmic domain. Because it is demonstrated that TRPV4 serine residue 824 (S824) is phosphorylated by serum/glucocorticoid regulated kinase 1, we elucidate the effect of TRPV4 S824 phosphorylation on TRPV association with PGM1. Even an inactivated mutant version of TRPV4, S824A, exhibited a decreased ability to bind PGM1, an activated phosphomimetic mutant version of TRPV4, S824D, exhibited enhanced binding to PGM1. Thus, formation of the TRPV4/PGM1 complex and localization of this complex to the plasma membrane appear to be regulated by the phosphorylation status of residue S824 in TRPV4. The newly identified interactor of TRPV4 may help the molecular pathways modulating transport activity or glucose metabolism, respectively.

Membrane Localization, Phosphoglucomutase1, Phosphorylation, Protein-Protein Interaction, TRPV4

Shin, S. , Lee, E. , Hyun, S. and Kang, S. (2016) Phosphorylation on TRPV4 Serine Residue 824 Enhances Its Association with PGM1. American Journal of Molecular Biology, 6, 33-44. doi: 10.4236/ajmb.2016.61004.