Author(s)

Xianyong Ma^1,2,3,4,5, Zongyong Jiang^1,2,3,4,5, Jian Zhang^1,2,3,4,5, Youjun Hu^1,2,3,4,5, Kaiguo Gao^1,2,3,4,5, Li Wang^1,2,3,4,5, Xuefen Yang^1,2,3,4,5

¹Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
²Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangzhou, China.
³State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, China.
⁴Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, China.
⁵Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, China.

The antioxidant and protective properties of a synthetic soybean isoflavone (ISO) were investigated using sow mammary gland cell. Cells were cultured with 10, 20 and 30 uM ISO, respectively, under 80 uM FeSO₄·7H₂O/H₂O₂ conditions. After 48 h of incubation, the cells in the presence of ISO were lost less compared with that of control under oxidative damage by H₂O₂/FeSO₄; ISO decreased the cell number at G1 and G2 stages, increased the cell number at S stage (all P < 0.05), it also reduced apoptosis of the cells (P < 0.01, P < 0.01, P < 0.05). The addition of ISO significantly promoted cell proliferation (P < 0.05) from 3^rd to 6^th days. Upon these, the activities of total superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant ability (T-AOC) also were increased and the activities of NADPH oxidase (NOX) decreased by ISO treatment (all P < 0.05). ISO decreased the concentration of ROS (P < 0.05) and MDA (P < 0.05). 10 uM, 20 uM, 30 uM ISO increased the relative mRNA abundance of SOD1 (all P < 0.05) and SOD2 (P < 0.05, P < 0.01, P < 0.05) and SOD3 (all P < 0.05); ISO significantly increased the relative mRNA abundance of GPX4 (all P < 0.01) and NOX4 (all P < 0.05), 20 uM ISO also increased the relative mRNA abundance of NOX2 (P < 0.05). It was concluded that supplementation of ISO enhanced the anti-oxidative function and prevented lipid peroxidation, possibly through the activation of the antioxidant enzymes and inhibition of cell apoptosis.

Isoflavone, Antioxidant System, Sow Mammary Gland Cell

Ma, X. , Jiang, Z. , Zhang, J. , Hu, Y. , Gao, K. , Wang, L. and Yang, X. (2015) Isoflavone Ameliorates H₂O₂ Induced Injury by Activating the Antioxidant System of Sow Mammary Gland Cell. Natural Science, 7, 571-580. doi: 10.4236/ns.2015.712057.