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Isoflavone Ameliorates H2O2 Induced Injury by Activating the Antioxidant System of Sow Mammary Gland Cell

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DOI: 10.4236/ns.2015.712057    4,147 Downloads   4,497 Views   Citations
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Xianyong Ma1,2,3,4,5, Zongyong Jiang1,2,3,4,5, Jian Zhang1,2,3,4,5, Youjun Hu1,2,3,4,5, Kaiguo Gao1,2,3,4,5, Li Wang1,2,3,4,5, Xuefen Yang1,2,3,4,5

ABSTRACT

The antioxidant and protective properties of a synthetic soybean isoflavone (ISO) were investigated using sow mammary gland cell. Cells were cultured with 10, 20 and 30 uM ISO, respectively, under 80 uM FeSO4·7H2O/H2O2 conditions. After 48 h of incubation, the cells in the presence of ISO were lost less compared with that of control under oxidative damage by H2O2/FeSO4; ISO decreased the cell number at G1 and G2 stages, increased the cell number at S stage (all P < 0.05), it also reduced apoptosis of the cells (P < 0.01, P < 0.01, P < 0.05). The addition of ISO significantly promoted cell proliferation (P < 0.05) from 3rd to 6th days. Upon these, the activities of total superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant ability (T-AOC) also were increased and the activities of NADPH oxidase (NOX) decreased by ISO treatment (all P < 0.05). ISO decreased the concentration of ROS (P < 0.05) and MDA (P < 0.05). 10 uM, 20 uM, 30 uM ISO increased the relative mRNA abundance of SOD1 (all P < 0.05) and SOD2 (P < 0.05, P < 0.01, P < 0.05) and SOD3 (all P < 0.05); ISO significantly increased the relative mRNA abundance of GPX4 (all P < 0.01) and NOX4 (all P < 0.05), 20 uM ISO also increased the relative mRNA abundance of NOX2 (P < 0.05). It was concluded that supplementation of ISO enhanced the anti-oxidative function and prevented lipid peroxidation, possibly through the activation of the antioxidant enzymes and inhibition of cell apoptosis.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Ma, X. , Jiang, Z. , Zhang, J. , Hu, Y. , Gao, K. , Wang, L. and Yang, X. (2015) Isoflavone Ameliorates H2O2 Induced Injury by Activating the Antioxidant System of Sow Mammary Gland Cell. Natural Science, 7, 571-580. doi: 10.4236/ns.2015.712057.

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