Chitinolytic Assay and Identification of Bacteria Isolated from Shrimp Waste Based on 16S rDNA Sequences - Advances in Microbiology

AiM > Vol.5 No.7, July 2015

Advances in Microbiology

Volume 5, Issue 7 (July 2015)

ISSN Print: 2165-3402 ISSN Online: 2165-3410

Google-based Impact Factor: 1.18 Citations h5-index & Ranking

Chitinolytic Assay and Identification of Bacteria Isolated from Shrimp Waste Based on 16S rDNA Sequences ()

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Download as PDF (Size: 535KB) PP. 541-548

DOI: 10.4236/aim.2015.57056 5,248 Downloads 7,899 Views Citations

Author(s)

Imanda N. Setia, Suharjono

Affiliation(s)

Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia.

ABSTRACT

Shrimp waste contains 20% - 60% chitin and possible to be source of chitinolytic bacteria. Chitinolytic bacteria are capable of hydrolyzing of chitin progressively to produce N-acetylglucosamine monomer which can be used to overcome the shrimp waste. The objectives of this research were to identify species of bacteria with high activity of chitin degradation in shrimp waste and to analyze their potency as chitin degradation agent. The research consists of screening of chitinolytic bacteria based on chitinolytic index, activity assay of chitinase using colorimetric method, and molecular identification of bacteria based on 16S rDNA sequences. Two of eighteen isolates of chitinolytic bacteria (PBK 2 and SA 1.2 isolates) showed the highest chitinolytic index, which were 2.069 and 2.084, whereas chitinase activity was 0.213 and 0.219 U/ml respectively. Based on 16S rDNA sequences, isolate of PBK 2 was identified as Acinetobacter johnsonii 3-1, whereas SA 1.2 was identified as Bacillus amyloliquefaciens GR53 with 99.78% similarity.

KEYWORDS

Chitinolytic Bacteria, Chitin, Shrimp, Shrimp Waste

Share and Cite:

Setia, I. and , S. (2015) Chitinolytic Assay and Identification of Bacteria Isolated from Shrimp Waste Based on 16S rDNA Sequences. Advances in Microbiology, 5, 541-548. doi: 10.4236/aim.2015.57056.

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