Author(s)

Victor M. Petrone-Garcia^1*, Raquel López-Arellano², Inkar Castellanos-Huerta³, Saeed El-Ashram⁴, Ebtesam Al-Olayan⁵, Danielle Graham³, Guillermo Tellez-Isaias³

¹Departamento de Ciencias Pecuarias, Universidad Nacional Autónoma de México (UNAM), Cuautitlan Izcalli, Estado de México, México.
²Laboratorio No 5: LEDEFAR, Unidad de Investigacion Multidisciplinaria, Facultad de Estudios Superi-ores Cuautitlan, UNAM, Cuautitlan Izcalli, Estado de México, México.
³Department of Poultry Science, University of Arkansas, Fayetteville, USA.
⁴Department of Zoology, Faculty of Science, Kafrelsheikh University, Kafr El-Sheikh, Egypt.
⁵Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.

This pilot study reports the vertical transmission and reverse thermosensitivity of the MS-H vaccine strain of Mycoplasma synoviae (MS) by RAPD in commercial breeders and their progeny. At two weeks of age, breeders were vaccinated with the ts⁺ MS-H strain. At 9 weeks of age, an outbreak of infectious synovitis (IS) was detected in the progeny. Tracheal swab samples were collected from breeders at 24, 39, 48, and 70 weeks of age. At 9 weeks, pullets swab from the elbow joints were collected. RAPD was performed on the isolates at 39.5°C, and the same ts^- MS-H strains were identified in the breeder hens and their progeny. Tracheal swabs from breeder hens were negative to MS isolation at 37°C and 39.5°C at 24- and 39-weeks. MS isolation was recovered from tracheal swabs from 9/10 and 10/10 breeders at 48- and 70- week. At 9 weeks of age in the progeny, MS was isolated from tracheal swabs of 10/10 from non-IS pullets. MS was isolated from 9/10 joints samples. The isolates from breeder hens and their progeny showed non-significant differences in five antimycoplasmic MIC100 values; otherwise, enrofloxacin presented a significant difference in MIC100 value (p < 0.05). This investigation demonstrated the reversal of the thermosensitivity, pathogenicity, and vertical transmission of the MS-H strain. Consequently, it is crucial to contemplate the danger of reversing pathogenicity and transmission to progeny when applying the MS-H vaccine strain.

Vertical Transmission, Thermosensitivity, Mycoplasma synoviae, RAPD, Vax-Safe®

Petrone-Garcia, V. , López-Arellano, R. , Castellanos-Huerta, I. , El-Ashram, S. , Al-Olayan, E. , Graham, D. and Tellez-Isaias, G. (2023) Isolation and Progeny Transmission of Non-Temperature-Sensitive MS-H Vaccine Strains of Mycoplasma synoviae from Temperature-Sensitive MS-H-Vaccinated Laying Breeder Hens. Food and Nutrition Sciences, 14, 579-588. doi: 10.4236/fns.2023.147038.