Author(s)

Rajesh Chakraborty, Sriparna Chatterjee, Sandipan Sarkar, Pabitra Chattopadhyay

Department of Chemistry, Burdwan University, Burdwan, India.
Durgapur Institute of Advanced technology and Management, Durgapur, India.
Institute of Physics, Bhubaneswar, India.

Hydrogen titanate nanotubes were synthesized by hydrothermal process using 10 M NaOH and TiO₂ anatase powder. The material synthesized was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to ensure the structural and morphological characteristics. The interaction of calf thymus DNA (CT-DNA) and bovine serum albumin protein with suspended aqueous solution of titanate nanotubes was investigated using UV absorption spectroscopy and the apparent association constant was found to be, K_b= 1.68 × 10⁴ M^-1 and K_ap=5.41 × 10³ M^-1 for DNA and BSA respectively. Addition of the titanate nano material resulted quenching of fluorescence spectra of ethidium bromide-DNA in tris HCl buffer solution and that of aqueous protein solution. The apparent binding constant (K_sv= 5.46 × 10⁴M^-1 for DNA binding and K_sv = 6.063 × 10³M^-1 for protein binding) was deduced from relevant fluorescence quenching data using Stern-Volmer equation.

Photoinduced Interaction; Nanotubes; Hydrothermal Process

R. Chakraborty, S. Chatterjee, S. Sarkar and P. Chattopadhyay, "Study of Photoinduced Interaction between Calf Thymus-DNA and Bovine Serum Albumin Protein with H₂Ti₃O₇ Nanotubes," Journal of Biomaterials and Nanobiotechnology, Vol. 3 No. 4, 2012, pp. 462-468. doi: 10.4236/jbnb.2012.34047.