Electronic Detection of Escherichia coli O157︰H7 Using Single-Walled Carbon Nanotubes Field-Effect Transistor Biosensor

Xiaoxian Zhang, Dongwei Wang, Danna Yang, Sai Li, Zhiqiang Shen

Academy of Military Medical sciences, Chinese PLA Center for Disease Control & Prevention, Tianjin, China.
School of Chemical Engineering, Sichuan University, Chengdu, China.

DOI: 10.4236/eng.2012.410B024                

Abstract

Field effect transistors (FET) based on Single-Walled Carbon Nanotubes (SWNTs) become the hot topic in fields of nano-electronic, clinical diagnostics, environmental testing etc. in recent years. In this paper, we reported a simple, scalable way to enrich semiconducting SWNTs by using HNO₃/H₂SO₄. Then carbon nanotube field-effect transistors (CNTFET) biosensor was fabricated with the enrichment SWNTs for Escherichia coli O157︰H7 detection. The response of each CNTFET was monitored in real time before and after introduction of the Escherichia coli O157︰H7 at various concentrations. The results show that CNT-FET biosensors we fabricated are sensitive to change of concentration of solution and response time is really short.

Keywords

Single-walled Carbon Nanotubes (SWNTs); Field Effect Transistors; Biosensor; Escherichia Coli O157︰H7

Conflicts of Interest

The authors declare no conflicts of interest.

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