Expression and localization of prostaglandin receptors and stromal factors in human cervix—Variations in pregnant and non-pregnant states
Chellakkan S. Blesson, Nathalie Roos, Olof Stephansson, Britt Masironi, Susann Reinert, Ylva Vladic Stjernholm, Gunvor Ekman-Ordeberg, Lena Sahlin
Division for Reproductive Endocrinology and the Pediatric Endocrinology Unit, Department of Women’s and Children’s Health, Karolinska University Hospital-Solna, Karolinska Institutet, Stockholm, Sweden.
Division for Reproductive Endocrinology and the Pediatric Endocrinology Unit, Department of Women’s and Children’s Health, Karolinska University Hospital-Solna, Karolinska Institutet, Stockholm, Sweden; Division of Obstetrics and Gynecology, Department of Women’s and Children’s Health, Karolinska University Hospital-Solna, Karolinska Institutet, Stockholm, Sweden.
Division of Obstetrics and Gynecology, Department of Women’s and Children’s Health, Karolinska University Hospital-Solna, Karolinska Institutet, Stockholm, Sweden.
Division of Obstetrics and Gynecology, Department of Women’s and Children’s Health, Karolinska University Hospital-Solna, Karolinska Institutet, Stockholm, Sweden; Clinical Epidemiology Unit, Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
DOI: 10.4236/ojmip.2013.34020   PDF   HTML     3,725 Downloads   6,783 Views   Citations


Prostaglandins (PGs) mediate cervical ripening leading to parturition. PGs are used successfully to induce cervical ripening. However, the cell type specific expression of PG receptor subtypes and various stromal factors important for cervical ripening in human cervix is not known. Our objective was to investigate the expression and localization of PG receptors EP1-4 and FP and localization of stromal factors CTGF (connective tissue growth factor), furin, calgranulin  B, ALOX12 (arachidonate 12-lipooxy-genase) and ALOX15 in human cervical tissue from pregnant and non-pregnant women. Cervical biopsies were obtained from non-pregnant (NP), term pregnant (TP) and post-partum (PP) women. The mRNA expression was determined with real-time PCR, protein expression and localization with immunohistochemistry. Our results show that the EP2 mRNA level was higher in the PP group as compared to TP, whereas the EP4 mRNA level was lower in the TP group as compared to NP. Concomitantly stromal EP2 and epithelial EP3 immunoreactivity was higher in the TP as compared to the NP group, while the EP4 immunostaining in glands was lower in the TP as compared to the PP group. Immunostaining of endothelial CTGF, smooth muscle furin and ALOX12, were all lower in the TP group as compared to NP, for CTGF also the PP group was lower than NP. Endothelial calgranulin B

immunoreactivity was higher in the PP group than the NP group. PG receptors and stromal factors exhibit differential expression in the cervix from women in non-pregnant and pregnant states, implying their involvement in the process of cervical ripening.

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S. Blesson, C. , Roos, N. , Stephansson, O. , Masironi, B. , Reinert, S. , Vladic Stjernholm, Y. , Ekman-Ordeberg, G. and Sahlin, L. (2013) Expression and localization of prostaglandin receptors and stromal factors in human cervix—Variations in pregnant and non-pregnant states. Open Journal of Molecular and Integrative Physiology, 3, 147-157. doi: 10.4236/ojmip.2013.34020.

Conflicts of Interest

The authors declare no conflicts of interest.


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