TITLE:
Rapid HPLC Method for Monitoring Relevant Residues of Pharmaceuticals Products in Environmental Samples
AUTHORS:
Juana Rodríguez Flores, Ana Maria Contento Salcedo, Lorena Muñoz Fernández
KEYWORDS:
High-Performance Liquid Chromatographic (HPLC), Breast Cancer, Antidepressant, Environmental Samples
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.2 No.1,
February
25,
2011
ABSTRACT: This work presents a multi-residue analytical method based on solid phase extraction (SPE) followed by high-performance liquid chromatographic (HPLC) with diode array (DAD) detection for the simultaneous determination of a group of pharmaceutical products that include ten antidepressants and three anticanceri- genic in environmental samples (water and soil). Baseline separation of the studied compounds was obtained on an ultrabase C18 (4.6 mm i.d. × 150 mm, 5 μm particle) column using acetonitrile:phosphate buffer pH 2.5 (35:65 v/v) as mobile phase with a flow rate of 1.5 mL/min. Different aspects including linearity, accuracy, precision and detection and quantification limits were examined in order to validate the proposed method. Detection limits between 1 and 50 ng/mL were obtained for all the target compounds. This method was ap- plied to the analysis of environmental samples as waters and soils of different precedence. Prior, the HPLC determination the samples were purified and enriched using SPE or liquid-liquid extraction (LLE) of the tar- get compounds.