TITLE:
The curative effect of the associated cell transplantation on the rabbit myocardial infarction
AUTHORS:
Zhicheng Fang, Chang’e Zhou, Xiang Zheng, Boyi Liu, Li Chen, Chunfeng Shen, Pei Liu, Yunfei Huang
KEYWORDS:
Mesenchymal Stem Cell; Cardiomyocycte-Like Cell; Endothelial Progenitor Cell; Cell Transplantation
JOURNAL NAME:
Stem Cell Discovery,
Vol.3 No.4,
October
7,
2013
ABSTRACT: Inducing Mesenchymal
stem cells to differentiate into cardiomyocycte-like cells and endothelial
progenitor cells orientedly and evaluating the curative effect of the
associated cell transplantation on the rabbit myocardial infarction (MI). Methods: Mesenchymal stem cells (MSCs)
were isolated from the bone marrow of 24 rabbits and cultured in special cell
culture medium containing 5-azacytidine
(5-AZA), endothelial cell growth supplements (ECGS), vascular endothelial
growth factor (VEGF) and basic fibroblast growth
factor (BFGF) respectively. The cell transplantation was performed 2
weeks after MI. Rabbits were divided into control group, cardiomyocytes-like
cell group, endothelial progenitor cell group
and combination group. We used the echocardiography to measure the heart
function 2 to 4 weeks after MI, TTC to measure the area of the infarction, flow
cytometry to estimate the cell apoptosis. Results: After induced, MSCs were differentiated orientedly into cardiomyocycte-like cells (CLCs)
and endothelial progenitor cells (EPCs). CLCs became greater and had a “stick”
or “ball” shape. Transmisson electron microscopy showed that the cells had oval
nuclei positioned in the central part and
well organized myofilaments, atrial granules and mitochomdrion. RT-PCR
showed the expression of the atrial natriuretic polypeptide, phospholamban and
myosin heavy chain in CLCs. EPCs formed confluent one-celled layer which showed
a cobblestone shape by phase-contrast microscope. The expression of CD133 in
EPCs was much at first and then descended gradually. Compared with the control
group, cell transplantation could improve the heart function, reduce the size
of MI, decrease the left ventricular end systole diameter and end diastolic
diameter, suppressed cell apoptosis. The curative effect of cell transplantation
was better in the associated-cell group than
in the single-cell transplantation group (LVEF: 32.49% ± 1.29% vs 53.22% ± 2.13% vs 56.91% ± 2.04% vs
62.61% ± 2.37%, P 0.04 vs 0.98 ± 0.12 vs 1.11 ± 0.03, P 0.02 vs 1.21 ± 0.09
vs 1.01 ± 0.01, P .98% vs 28.61% ± 1.24% vs 29.73% ± 2.11% vs 22.82%
± 3.12%, P % vs 6.94% ± 0.59% vs 6.4% ± 0.27% vs 4.63% ±
0.74%, P clusions: This study showed that MSCs can differentiate into CLCs and EPCs in the given conditions and the associated cell transplantation
is better than the single cell transplantation
to treat MI.