TITLE:
Evaluation of the Effects of Corticosteroids on Histamine Release by ex Vivo Cutaneous Microdialysis
AUTHORS:
Carol Courderot-Masuyer, Sophie Robin, Hélène Tauzin, Sylvain Harbon, Sophie Mac-Mary, Alexandre Guichard, Patrice Muret, Philippe Humbert
KEYWORDS:
MacKenzie Test; Corticosteroids; ex Vivo Cutaneous Microdialysis; Histamine
JOURNAL NAME:
Journal of Cosmetics, Dermatological Sciences and Applications,
Vol.3 No.3,
September
25,
2013
ABSTRACT: The purpose of the study was to evaluate the
effects of corticosteroids on histamine release and to compare their potency
with the MacKenzie classification based on their vasoconstrictor effects. Thanks to ex Vivo cutaneous microdialysis, we studied
histamine-induced release over a period of time on excised abdominal skin from
women. Eight corticosteroids were topically applied with occlusive dressing
onto the skin, above probes, before anti-IgE injection. Histamine levels were
assessed by an EIA method. In order to compare the different corticosteroids,
AUC was calculated allowing an estimation of the amount of released histamine
for 60 min of ex vivo cutaneous
microdialysis. Diflucortolone
0.1% and micronized betamethasone dipropionate 0.05% are considered as corticosteroids
with high potency in MacKenzie classification. Betamethasone dipropionate
associated with propylene glycol 0.05%, belongs to a stronger
class in Mackenzie classification. Our results showed that the decrease in
histamine release was more important with difluocortolone than with both of these corticosteroids. Therefore there was no correlation between the vasoconstrictor potency
of topical corticosteroids and their ability to inhibit histamine release.