TITLE:
Molecular Genetic Diversity in Iranian Populations of Puccinia triticina, the Causal Agent of Wheat Leaf Rust
AUTHORS:
Seyed Taha Dadrezaie, Samer Lababidi, Kumarse Nazari, Ebrahim Mohammadi Goltapeh, Farzad Afshari, Fida Alo, Masoud Shams-Bakhsh, Naser Safaie
KEYWORDS:
Leaf Rust; Gene Resistance; Genetic Diversity; Puccinia triticina; AFLP and FAFLP Markers
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.4 No.7,
July
5,
2013
ABSTRACT:
Wheat leaf rust caused by Puccinia triticina,
is the most common and widely distributed wheat rust in the world. In order to study the genetic structure
of leaf rust population 14 pairs of AFLP and 6 pairs of FAFLP primers evaluated on 86 isolates samples collected in Iran during spring
of 2009. Results showed that almost all investigated isolates were genetically different
and special pattern of AFLP allele’s that confirm high genetic diversity within
leaf rust population was observed. Analyses showed, all provinces were classified
into three major groups particularly similar clusters were found between then neighboring
provinces. Rust spore can follow the migration pattern in short and long distances
to neighbor in provinces. Results indicated that the greatest variability was revealed
by 97% of genetic differentiation within leaf rust populations and the lesser variation
of 3% was observed between the rust populations. These results suggested that each
population was
not completely identical and high gene flow has occurred among the leaf rust population
of different provinces. The highest differentiation and genetic distance among the
Iranian leaf rust populations was detected between leaf rust population in Sistan
and Baluchistan and highest similarity was observed between in Ardabil provinces. The high pathogenic variability of leaf
rust races in Ardabil and Northern Khorasan may
be an indication that
these two regions are the center of origin of pathogenic arability. Present study
shows that leaf rust population in Iran is highly dynamic and variable.