L. Y. Liang, Z. Feng, J. Yesuf and J. W. Blackburn, “Optimization of Growth Medium and Enzyme Assay Conditions for Crude Cellulases Produced by a Novel Thermophilic and Cellulolytic Bacterium Anoxybacillus sp. 527.” Applied Biochemistry and Biotechnology, Vol. 160, No. 6, 2010, pp. 1841-1852. doi:10.1007/s12010-009-8677-x

has been cited by the following article:

• TITLE: Optimization of Cultural Condition and Synergistic Effect of Lactose with Carboxymethyl Cellulose on Cellulase Production by Bacillus sp. Isolated from Fecal Matter of Elephant (Elephas maximus)

  AUTHORS: Sangrila Sadhu, Pallab Kumar Ghosh, Tarun Kumar De, Tushar Kanti Maiti

  KEYWORDS: Avicelase; β-Glucosidase; Carboxymethyl Cellulase (CMCase); Lactose

  JOURNAL NAME: Advances in Microbiology, Vol.3 No.3, July 3, 2013

  ABSTRACT: A cellulase producing bacterium (E3 strain) was isolated from fecal matter of elephant and identified as Bacillus sp. using 16S rDNA sequenced based molecular phylogenetic approach. While studying the effect of substrates like Carboxymethyl cellulose (CMC), avicel, starch, maltose, sucrose, glucose, fructose, galactose and lactose on cellulase production, it was found that CMC was best carbon source induced cellulase production followed by lactose in this bacterial strain. A positive synergistic effect of lactose with CMC was also observed with enhancement of 5 - 6 times in cellulase production. The optimum cellulase production was recorded with 1% CMC and 1% lactose when added individually in the Omeliansky’s medium. The results showed that addition lactose with CMC greatly enhances the production and activity of various cellulase enzymes. The optimal fermentation conditions for the biosynthesis of cellulase by this strain were found to be temperature: 37℃, pH 7.0. The nitrogen source NH4Cl at 0.15% was optimum for cellulase production by this bacterium.