TITLE:
Establishment and molecular characterization of human dermal mesenchymal-like stem cells derived from human scalp biopsy of androgenetic alopecia patient
AUTHORS:
Pravin Potdar, Kavitha S. Kumar
KEYWORDS:
Dermal Cells; Alopecia; Mesenchymal Stem Cells; Keratin 18; CD34; CD105
JOURNAL NAME:
Stem Cell Discovery,
Vol.3 No.2,
April
17,
2013
ABSTRACT: Development
of Dermal cell line has great scope in the field of skin related diseases and
regenerative medicine. Alopecia leads to a skin disorder causing balding and
its mechanism is not yet understood. In the present study, we have developed
and characterized a heterogeneous population of human dermal mesenchymal-like
stem cell line from scalp biopsy of androgenetic alopecia patient with a view
to isolate cells from the bulge region of the hair follicle. Our study showed
that the dermal cells isolated from dermis of skin showed epithelial-like cells
expressing CD34 and Keratin 18, which are characteristic of bulge hair follicle
cells. These cells also expressed mesenchymal phenotypes and pluripotency
markers such as Oct4, Nanog and SOX2. These cells were designated as
“Human Dermal Mesenchymal-like Stem Cells (hDMSCs)”. To confirm their
epithelial phenotypes, we have grown these cells at low serum concentration and
it was observed that 3% serum concentration provided optimum conditions for
their growth and maintenance of characteristics. The hDMSCs cells are presently
at passage 10. This study reports the establishment of human dermal
mesenchymal-like cell line from the dermis of Alopecia patient, which may be
used as an in vitro model system to study the mechanism of Alopecia and other related skin
disorders.