X. Liu, Y. Zhu and S. T. Yang, “Construction and Characterization of Ack Deleted Mutant of Clostridium Tyrobutyricum for Enhanced Butyric Acid and Hydrogen Production,” Biotechnology Progress, Vol. 22, No. 5, 2006, pp. 1265-1275. doi:10.1021/bp060082g

has been cited by the following article:

• TITLE: Metabolic Engineering of Thermoanaerobacterium thermosaccharolyticum for Increased n-Butanol Production

  AUTHORS: Ashwini Bhandiwad, Anna Guseva, Lee Lynd

  KEYWORDS: Biofuels; n-Butanol; Thermophile; Metabolic Engineering

  JOURNAL NAME: Advances in Microbiology, Vol.3 No.1, March 27, 2013

  ABSTRACT: Thermoanaerobacterium thermosaccharolyticum shows promise as a host for n-butanol production since it natively has the required genes involved in the n-butanol biosynthetic pathway. Overexpression of the natively occurring bcs operon containing the genes thl, hbd, crt, bcd, etfA, and etfB responsible for the formation of butyryl CoA increased the n-butanol production by 180% compared to the wild type from a n-butanol titer of 1.8 mM to 5.1 mM. The deletion of one of the six alcohol dehydrogenase genes confirmed that it was the primary gene responsible for ethanol and n-butanol production from acetyl CoA and butyryl CoA respectively.