TITLE:
Novel strategy to diagnose and grade hepatocellular carcinoma
AUTHORS:
Shahida Parveen, Riffat Mehboob, Asad Aslam Khan, Mulazim Hussain Bukhari, Fridoon Jawad Ahmad
KEYWORDS:
P53; AgNOR; Hepatocellular Carcinoma; Cirrhosis; Immunohistochemistry
JOURNAL NAME:
Natural Science,
Vol.5 No.1,
January
14,
2013
ABSTRACT: Hepatocellular carcinoma (HCC) is among the most common malignancies worldwide, particularly in South and South East Asia. Unfortunately due to lack of appropriate facilities and awareness only limited information is available about its early diagnosis. Aim of the present study was to determine the efficacy of p53 by immunohistochemistry and Argyrophilicnucleolar organizer regions (AgNORs) in diagnosis of HCC and cirrhosis of liver. A total of 100 liver biopsies were studied, it included 20 cases of HCC, 60 cases of cirrhosis of the liver and 20 cases of normal liver from autopsy specimens as a control. Out of 20 cases of HCC, 15 were positive for p53 stain and 5 were negative. None of the 60 cases of cirrhosis or 20 with normal histology revealed p53 expression. A statistically significant (p . It helps in differentiation from well differentiated to moderately and to poorly differentiated HCC.