TITLE:
Development of H5 subtype-specific monoclonal antibodies (MAb) and MAb-based assays for rapid detection of H5 avian influenza
AUTHORS:
Huaguang Lu, Lin Lin, Ronghui Wang, Yanbin Li, Yanbin Li, Bill Scheuchenzuber, Jiabo Liu, Zhiqin Xie, Joseph A. Rosebrock
KEYWORDS:
Avian Influenza Virus; Hemagglutinin; Hybridoma Cell Line, Monoclonal Antibodies; Dot-ELISA; Biosensor
JOURNAL NAME:
Health,
Vol.4 No.10A,
November
1,
2012
ABSTRACT: Avian influenza (AI) virology surveillance is the most important method to monitor AI virus (AIV) in poultry so as to effectively prevent and control AI outbreaks. Monoclonal antibodies (MAb)-based assays are highly sensitive and specific for AIV detection, and much practical and economic for test-in-field or onsite. Many such assays have been developed and are still in developing since the H5N1 highly pathogenic AI (HPAI) outbreaks occurred in South East Asia in 2003. A MAb-based dot-enzyme-linked immunosorbent assay (ELISA) has been developed in our lab during late 1990s and early 2000s. Meanwhile, AIV H7 and H5 subtype specific-MAbs have been successfully developed in our laboratory to enhance the Dot-ELISA and other MAb-based assays for AIV detection. Production and purification of the H7 and H5 MAbs were made to provide essential reagents for Dot-ELISA and other immunoassays, and the current development of a novel Biosensor technique for rapid detection of AIV from clinical and field specimens.