TITLE:
Identification and Isolation of Constituents Contained in Venoms and Plant Extracts
AUTHORS:
Doreen Stacy Palu
KEYWORDS:
Ilex aquifolium Linne, Calicotome villosa (Poiret) Link, 13C-NMR Identification, 1H-NMR Quantitation, Dendroaspis angusticeps, Fasciculin II, Naja pallida, Cardiotoxin
JOURNAL NAME:
Natural Resources,
Vol.16 No.13,
December
29,
2025
ABSTRACT: The present paper is part of publication of the proceedings of the international symposium “Tropical Islands and Biodiversity”. The aim is to place emphasis on natural products from plant and animal biodiversity. It also emphazises methods and techniques widely commonly used to identify and isolate components from natural complex mixtures, through two studies. First study is about natural products of animal origin. It presents steps leading to isolation of two high-valued native toxins: Fasciculin-II and Cardiotoxin isolated from Dendrosaspis angusticeps and Naja pallida venoms, respectively. Second study describes chemical composition of wild growing corsican understudied plant species, with potential biological activities. Phytochemical constituents from Ilex aquifolium Linne leaves and berries extracts have been first determined. Among identified compounds, ursolic and oleanolic acids displaying therapeutic interests in various domains have also been quantified by 1H-NMR in a crude leaves extract using a reliable method developed and validated. Then, phytochemical constituents from Calicotome villosa (Poiret) Link flowers and roots extracts have been determined. Finally, plant extracts and identified secondary metabolites were evaluated for their antimicrobial activities.