TITLE:
Histopathological and Molecular Aspects of Women’s Breast Cancer in Bouaké: Preliminary Study on 43 Cases
AUTHORS:
Faїza Alassani, Vincent Yapo, Lazare Touré, Issouf Bamba, Sarhatou Kamara, Fatoumata Koné, Ibrahiman Touré, Yaya Samaké, Dagoun Elysée Boko, Darya Kizub, Kouamé Justin N’dah
KEYWORDS:
Breast, Cancer, Pathology, BRCA, Bouaké
JOURNAL NAME:
Open Journal of Pathology,
Vol.15 No.4,
October
30,
2025
ABSTRACT: The objective of the current study was to determine the expression profiles of the Breast Cancer genes 1 and 2, two tumor suppressing genes, in patients with breast cancer in the Gbêkê region of Côte d’Ivoire. This cross-sectional study was carried from September 2023 to January 2025. The patients came from five screening and management sites for breast diseases in the Gbêkê region. Breast micro-biopsies were stored in an RNA Protect solution at −80˚C before processing. RNA extraction was performed with RNAEsy/Triazol kit from Qiagen. And RT-qPCR was carried out using specific primers and probes for BRCA1 and 2 mRNAs. Fifty-eight participants were recruited. Among which them, 43 had breast carcinoma and 15 had various non-malignant breast tumors. The average age of the participants was 42.9 ± 10.1 with ages ranging from 24 to 71 years. These women were mostly unemployed and/or not civil servants in 93% of the cases. Out of them, 9.3% used hormonal contraceptives, and 72.1% were premenopausal. The BRCA1 gene was expressed in 25.6% of carcinoma patients and 20.0% of the non-malignant participants. And its expression levels were low in both groups. The BRCA2 gene was expressed in 62.8% of carcinoma cases and 66.7% of the non-malignant cases. And its expression levels were high in both groups. Breast cancer is frequent in the Gbêkê region. It would therefore be wise to integrate the expression profile of BRCA1 and BRCA2 in all women from adolescence for rigorous monitoring of the disease.