TITLE:
Research on the Identification of Angelica Sinensis by PCR + Sequencing Based on ITS2
AUTHORS:
Fugang Xiao, Ruichen Ye, Jinqi Yang, Jiongyan Liu, Xiaodong Zhang, Pengfei Cui, Bailing Yin, Deguo Wang
KEYWORDS:
Angelica Sinensis, ITS2, PCR, Cloning, Nucleic Acid Comparison
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.13 No.7,
July
30,
2025
ABSTRACT: The adulteration of Chinese medicinal materials has become an important obstacle to the development of traditional Chinese medicine, and it is urgent to establish identification methods for Chinese medicinal materials. This project aims to establish a method for identifying the authenticity of Angelica sinensis based on the ITS2 gene. By extracting Angelica sinensis DNA, performing PCR amplification, adding an A-tail, nucleic acid electrophoresis, gel recovery, constructing a vector, transformation and blue white screening, plasmid extraction, PCR and enzyme digestion detection, and finally sequencing, the authenticity of Angelica sinensis samples is identified through gene library comparison. The identification results of 8 Angelica sinensis samples showed that 4 were Angelica sinensis, 3 were Angelica dahurica, and 1 was Kitagawia terebinthacea. The similarity between the ITS2 fragment and the gene library was 98.99%, 95.56%, and 98.99%, respectively. The gene identification method established in this study can accurately identify the authenticity of Angelica sinensis samples and provide a reference for Angelica sinensis identification.