TITLE:
Phytochemical Studies, Antimicrobial and Anti-Inflammatory Properties of the Hydroethanolic Extract of Kalanchoe pinnata (Lam.) Pers. from Togolese Flora
AUTHORS:
Luckman Gbati, Yao Hoinsou, Fo-Doh Clefasse Koula, Ablam Alognon, Minyo Ega Sossa Montant, Sossawè Poli, Tibanguebé Doumongue, Gérard Akouétévi Toudji, Efui Holaly Gbekley, Bouraïma Djeri, Aboudoulatif Diallo, Damintoti Simplice Karou
KEYWORDS:
Kalanchoe pinnata, Hydroethanolic Extract, Phytochemistry, Pharmacological Activities, Togo
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.12 No.10,
October
24,
2024
ABSTRACT: Kalanchoe pinnata (Lam.) Pers. is known as a plant that has many special benefits such as anti-inflammatory and antibacterial. The present study was carried out to perform a phytochemicals study and evaluate the antimicrobial and anti-inflammatory activity of the hydroethanolic extract of Kalanchoe pinnata (Lam.) Pers. leaves. After phytochemicals screening, the content of phenolic compounds, proanthocyanidol and flavonoids in the extract of this plant was determined spectrophotometrically. Antimicrobial activity was assessed using the micro-dilution technique on 96-well plates in liquid medium, combined with agar spreading. Anti-inflammatory activity was assessed using the 1% carrageenan induced rat paw oedema model. Phytochemical screening revealed the presence of alkaloids, saponins, triterpenes and sterols, phenols and flavonoids in the plant extract in varying proportions. The extract contained (0.049 ± 0.03 µg EAG/mg extract) total polyphenols, (0.215 ± 0.025 µg CE/mg extract) proanthocyanidins and (385.435 ± 0.0328 µg ER/mg ES) flavonoids. The hydroethanolic extract of the leaves of this plant inhibited the in vitro growth of the microbial strains studied to varying degrees. The MIC of the extract varied from 12.5 to 25 mg/mL and the BMC from 12.5 to 50 mg/mL. The plant did not show any activity on 1% carrageenan-induced rat paw edema.