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has been cited by the following article:

• TITLE: Optimization and Validation of a Simple Spectrophotometric Based DPPH Method for Analysis of Antioxidant Activity in Aerated, Semi-Aerated and Non-Aerated Tea Products

  AUTHORS: Simon Mwangi Kingori, Sylvester Cheruiyot, Aron Cheruiyot Kirui, Rugema Grace Uwamahoro, Agnes Wangui Mwangi

  KEYWORDS: UV-Spectrophotometric, Optimized, Validated, Antioxidant

  JOURNAL NAME: Open Journal of Applied Sciences, Vol.14 No.8, August 23, 2024

  ABSTRACT: In this study, the simple DPPH UV-spectrophotometric analytical procedures were optimized and validated for the estimation of antioxidant properties in aerated, semi-aerated and non-aerated tea products as per the AOAC guidelines. The method was found to be simple, rapid, accurate and economical in estimating antioxidant properties in the different tea products. A (0.0100 ± 0.0001) g well ground tea sample was extracted directly in 80% v/v methanol in distilled water. The precision of the method had an RSD% of 1.695 with a linear relation equation of Y = 200.7X + 5.25 (r2 = 0.997). The method proved to be a reliable and effective tool in analysis of anti-oxidant properties in varied tea cultivars and their processed products.