Klemola, K., Pearson, J., Von Wright, A., Liesivuori, J. and Lindström-Seppä, P. (2007) Evaluating the Toxicity of Reactive Dyes and Dyed Fabrics with the Hepa-1 Cytotoxicity Test. Autex Research Journal, 7, 224-230.
https://doi.org/10.1515/aut-2007-070307

has been cited by the following article:

• TITLE: Bioremediation of Textile Azo Dyes Amido Black 10B, Reactive Black 5, Reactive Blue 160 by Lentinus squarrosulus AF5 and Assessment of Toxicity of the Degraded Metabolites

  AUTHORS: Anshu Mathur, Chandrachur Ghosh, Partha Roy, Ramasare Prasad, Rajesh Pratap Singh

  KEYWORDS: Lentinus squarrosulus AF5, Azo Dyes, FTIR, 1H NMR, Catabolism, Cytotoxicity

  JOURNAL NAME: Advances in Microbiology, Vol.14 No.2, February 29, 2024

  ABSTRACT: Bioremediation is an eco-compatible and economical approach to counter textile dye menace. The isolated Lentinus squarrosulus AF5 was assessed for decolourization of textile azo dyes, and had shown ~93%, 88% and 70% decolorization of Reactive blue 160 (RB160), Reactive black 5 (RB5) and Amido black 10B (AB10B) respectively. Further analysis using UV-vis, HPLC, and FTIR, 1H NMR had shown the degradation of the dyes. Toxicity analysis of the metabolites was performed using seed germination and plant growth on two agriculturally important plants Guar (Cyamopsis tetragonoloba) and wheat (Triticum aestivum) as well as cytotoxicity analysis using the human keratinocyte cell line (HaCaT). The dye mix appeared inhibitory for seed germination (20% - 40%), whereas metabolites were non-inhibitory for germination. Treatment of HaCaT cells with of dye mix and metabolites led into 45% and ~100% of cell viability of HaCaT cells respectively. Therefore, metabolites following degradation of the dye mix were observed to be non-toxic.