TITLE:
Tyrosine Aminotransferase Gene (SmTAT) Revealed Genetic Diversity and Phylogeny of Cultivated Danshen (Salvia miltiorrhiza) Populations
AUTHORS:
Guanrong Li, Ruihua Ren, Deying Kong, Jie Feng, Yanyan Yin, Fang Liao
KEYWORDS:
Danshen (Salvia miltiorrhiza Bge), Cultivated Population, Tyrosine Aminotransferase Gene (TAT), Genetic Diversity, Phylogeny
JOURNAL NAME:
Natural Resources,
Vol.13 No.3,
March
23,
2022
ABSTRACT: Chinese traditional medicine Danshen is the
radix of the perennial herbs of Salvia miltiorrhiza Bunge, which has a
variety of pharmacological effects and is traditionally and extensively applied
clinically to treat cardiovascular disorders. In this research, the genomic
genes for tyrosine aminotransferase (TAT) of 38 cultivated populations
of Danshen in China were cloned and bioinformatic analyses were conducted to reveal its
genetic diversity and phylogeny. The full-length SmTAT was 2296 - 2444 bp including 6 exons (encoding 411 amino acids)
and 5 introns. Overall, the SmTAT genes in cultivated Danshen populations are highly conserved with a relative low level of genetic diversity. The spliced
exons (1236 bp) had 23 SNP variations with a rate of 1.86%, of which 22
occurred in the white flower S. miltiorrhiza Bge.f.alba population
(W-SCHY-W-1) and led to 5 amino acid variations. The entire 290 SNP variations
with a rate of 24% in the 5 introns occurred exclusively in W-SCHY-W-1.
Phylogenetic trees based on the full-length, combined introns, the
spliced exons, and the deduced amino acid sequences of SmTAT all showed
a two-clade basic structure with W-SCHY-W-1 uniquely standing alone. The SmTAT gene of the white flower population (W-SCHY-W-1) is unique and especially rich
in variations. The first time clarified genomic SmTAT gene structure and
genetic diversity in cultivated Danshen populations laid an excellent
foundation for further studies on the biosynthesis of bioactives and the molecular
breeding of Danshen as well as in plant tyrosine metabolism.