TITLE:
Flavonoids Reduce Lipid Peroxides and Increase Glutathione Levels in Pooled Human Liver Microsomes (HLMs)
AUTHORS:
William Yaw Boadi, Camille Stevenson, Dontrez Johnson, Mohamed Adel Mohamed
KEYWORDS:
Flavonoids, Glutathione (GSH), Human Liver Microsomes (HLMs), Lipid Peroxidation, Oxidative Stress
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.11 No.6,
December
15,
2021
ABSTRACT: The effects of each of the flavonoids; genistein
(G), quercetin (Q) and kaempferol (K) at several doses on lipid peroxides
(LP) and reduced glutathione (GSH) in pooled human liver microsomes (HLMs) were
investigated following the oxidative damage for 4, 6, 18 and 24 hr. HLMs (1
mg/ml) were exposed to each of the above flavonoids at 0, 5, 10, 15, 20 or 25
μM and incubated for the respective times as previously stated. Our hypothesis
was that HLMs exposed to the flavonoids for the respective exposure times can
decrease LP and increase GSH in HLMs to better cope with the oxidative stress. The results of our studies indicate that each of
the flavonoids significantly (p levels observed for Q for the
24-hr. incubation. The findings suggest that the flavonoids modulate oxidative
stress in HLMs by decreasing LP and such decreases in LPs may be due to the
increasing and or the replenished levels of GSH in the said cells to better
cope with the oxidative stress.